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Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion
A major unsolved issue of prion biology is the existence of multiple strains with distinct phenotypes and this strain phenomenon is postulated to be associated with the conformational diversity of the abnormal prion protein (PrP(Sc)). Real-time quaking-induced conversion (RT-QUIC) assay that uses Es...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601500/ https://www.ncbi.nlm.nih.gov/pubmed/26284507 http://dx.doi.org/10.1080/19336896.2015.1062201 |
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author | Sano, Kazunori Atarashi, Ryuichiro Nishida, Noriyuki |
author_facet | Sano, Kazunori Atarashi, Ryuichiro Nishida, Noriyuki |
author_sort | Sano, Kazunori |
collection | PubMed |
description | A major unsolved issue of prion biology is the existence of multiple strains with distinct phenotypes and this strain phenomenon is postulated to be associated with the conformational diversity of the abnormal prion protein (PrP(Sc)). Real-time quaking-induced conversion (RT-QUIC) assay that uses Escherichia coli-derived recombinant prion protein (rPrP) for the sensitive detection of PrP(Sc) results in the formation of rPrP-fibrils seeded with various strains. We demonstrated that there are differences in the secondary structures, especially in the β-sheets, and conformational stability between 2 rPrP-fibrils seeded with either Chandler or 22L strains in the first round of RT-QUIC. In particular, the differences in conformational properties of these 2 rPrP-fibrils were common to those of the original PrP(Sc). However, the strain specificities of rPrP-fibrils seen in the first round were lost in subsequent rounds. Instead, our findings suggest that nonspecific fibrils became the major species, probable owing to their selective growth advantage in the RT-QUIC. This study shows that at least some strain-specific conformational properties of the original PrP(Sc) can be transmitted to rPrP-fibrils in vitro, but further conservation appears to require unknown cofactors or environmental conditions or both. |
format | Online Article Text |
id | pubmed-4601500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-46015002016-02-03 Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion Sano, Kazunori Atarashi, Ryuichiro Nishida, Noriyuki Prion Extra View A major unsolved issue of prion biology is the existence of multiple strains with distinct phenotypes and this strain phenomenon is postulated to be associated with the conformational diversity of the abnormal prion protein (PrP(Sc)). Real-time quaking-induced conversion (RT-QUIC) assay that uses Escherichia coli-derived recombinant prion protein (rPrP) for the sensitive detection of PrP(Sc) results in the formation of rPrP-fibrils seeded with various strains. We demonstrated that there are differences in the secondary structures, especially in the β-sheets, and conformational stability between 2 rPrP-fibrils seeded with either Chandler or 22L strains in the first round of RT-QUIC. In particular, the differences in conformational properties of these 2 rPrP-fibrils were common to those of the original PrP(Sc). However, the strain specificities of rPrP-fibrils seen in the first round were lost in subsequent rounds. Instead, our findings suggest that nonspecific fibrils became the major species, probable owing to their selective growth advantage in the RT-QUIC. This study shows that at least some strain-specific conformational properties of the original PrP(Sc) can be transmitted to rPrP-fibrils in vitro, but further conservation appears to require unknown cofactors or environmental conditions or both. Taylor & Francis 2015-08-18 /pmc/articles/PMC4601500/ /pubmed/26284507 http://dx.doi.org/10.1080/19336896.2015.1062201 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Extra View Sano, Kazunori Atarashi, Ryuichiro Nishida, Noriyuki Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion |
title | Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion |
title_full | Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion |
title_fullStr | Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion |
title_full_unstemmed | Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion |
title_short | Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion |
title_sort | structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion |
topic | Extra View |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601500/ https://www.ncbi.nlm.nih.gov/pubmed/26284507 http://dx.doi.org/10.1080/19336896.2015.1062201 |
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