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Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection
The importance of transmission of occult HBV infection (OBI) via transfusion, organ transplantation and hemodialysis has been widely recognized. However, data regarding the transmission of OBI through close contact remain limited. In this study, serum samples were obtained from a child and his paren...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601727/ https://www.ncbi.nlm.nih.gov/pubmed/26457811 http://dx.doi.org/10.1371/journal.pone.0138552 |
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author | Hu, Li-Ping Liu, De-Ping Chen, Qin-Yan Harrison, Tim J. He, Xiang Wang, Xue-Yan Li, Hai Tan, Chao Yang, Qing-Li Li, Kai-Wen Fang, Zhong-Liao |
author_facet | Hu, Li-Ping Liu, De-Ping Chen, Qin-Yan Harrison, Tim J. He, Xiang Wang, Xue-Yan Li, Hai Tan, Chao Yang, Qing-Li Li, Kai-Wen Fang, Zhong-Liao |
author_sort | Hu, Li-Ping |
collection | PubMed |
description | The importance of transmission of occult HBV infection (OBI) via transfusion, organ transplantation and hemodialysis has been widely recognized. However, data regarding the transmission of OBI through close contact remain limited. In this study, serum samples were obtained from a child and his parents. The child had received the standard vaccination regimen at birth and produced protective antibody. Sera were tested for HBV serological markers. Nested PCR assays were used to detect HBV DNA and the amplicons were cloned and their sequences subjected to phylogenetic analysis. The results showed that both parents had occult infections while the child had an overt infection. Twelve, eleven and nine clones, from the father, mother and son, respectively, were sequenced. Serotypes adrq+, ayw1, ayw and ayr were found in the father and ayw1, adw2 and adwq+ in the mother; adrq+ was the only serotype in son. Genotype B, subgenotype C2 and a recombinant were identified in the father and genotype B, subgenotype C5 and three recombinants were found in the mother. Subgenotype C2 was the only genotype identified in the child. A phylogenetic tree showed that all of the child’s sequences and most of the father’s sequences clustered together. However, none of mother’s sequences clustered with those of the child. The surface gene from the child and his father had the same amino acid substitution pattern (T118K, T123N and G145A). We concluded that the father was the source of the son’s HBV infection, suggesting that occult HBV infection may be transmitted through close contact and manifest as an overt infection. |
format | Online Article Text |
id | pubmed-4601727 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46017272015-10-20 Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection Hu, Li-Ping Liu, De-Ping Chen, Qin-Yan Harrison, Tim J. He, Xiang Wang, Xue-Yan Li, Hai Tan, Chao Yang, Qing-Li Li, Kai-Wen Fang, Zhong-Liao PLoS One Research Article The importance of transmission of occult HBV infection (OBI) via transfusion, organ transplantation and hemodialysis has been widely recognized. However, data regarding the transmission of OBI through close contact remain limited. In this study, serum samples were obtained from a child and his parents. The child had received the standard vaccination regimen at birth and produced protective antibody. Sera were tested for HBV serological markers. Nested PCR assays were used to detect HBV DNA and the amplicons were cloned and their sequences subjected to phylogenetic analysis. The results showed that both parents had occult infections while the child had an overt infection. Twelve, eleven and nine clones, from the father, mother and son, respectively, were sequenced. Serotypes adrq+, ayw1, ayw and ayr were found in the father and ayw1, adw2 and adwq+ in the mother; adrq+ was the only serotype in son. Genotype B, subgenotype C2 and a recombinant were identified in the father and genotype B, subgenotype C5 and three recombinants were found in the mother. Subgenotype C2 was the only genotype identified in the child. A phylogenetic tree showed that all of the child’s sequences and most of the father’s sequences clustered together. However, none of mother’s sequences clustered with those of the child. The surface gene from the child and his father had the same amino acid substitution pattern (T118K, T123N and G145A). We concluded that the father was the source of the son’s HBV infection, suggesting that occult HBV infection may be transmitted through close contact and manifest as an overt infection. Public Library of Science 2015-10-12 /pmc/articles/PMC4601727/ /pubmed/26457811 http://dx.doi.org/10.1371/journal.pone.0138552 Text en © 2015 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hu, Li-Ping Liu, De-Ping Chen, Qin-Yan Harrison, Tim J. He, Xiang Wang, Xue-Yan Li, Hai Tan, Chao Yang, Qing-Li Li, Kai-Wen Fang, Zhong-Liao Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection |
title | Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection |
title_full | Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection |
title_fullStr | Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection |
title_full_unstemmed | Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection |
title_short | Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection |
title_sort | occult hbv infection may be transmitted through close contact and manifest as an overt infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601727/ https://www.ncbi.nlm.nih.gov/pubmed/26457811 http://dx.doi.org/10.1371/journal.pone.0138552 |
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