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Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite
OBJECTIVE: In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffold...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601863/ https://www.ncbi.nlm.nih.gov/pubmed/26464814 |
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author | Razavi, Shahnaz Karbasi, Saeed Morshed, Mohammad Zarkesh Esfahani, Hamid Golozar, Mohammad Vaezifar, Sedigheh |
author_facet | Razavi, Shahnaz Karbasi, Saeed Morshed, Mohammad Zarkesh Esfahani, Hamid Golozar, Mohammad Vaezifar, Sedigheh |
author_sort | Razavi, Shahnaz |
collection | PubMed |
description | OBJECTIVE: In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffolds was then evaluated. MATERIALS AND METHODS: In this experimental study, the PLGA/CS scaffolds were prepared at the different ratios of 90/10, 80/20, and 70/30 (w/w) %. Morphology, cell adhesion and prolif- eration rate of h-ADSCs on the scaffolds were assessed using scanning electron microscope (SEM), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and trypan blue staining respectively. RESULTS: H-ADSCs seeded on the matrices indicated that the PLGA/CS composite matrix with aligned nanofibres and higher content of CS nano-powders gave significantly better performance than others in terms of cell adhesion and proliferation rate (P<0.05). CONCLUSION: We found that CS enhanced cell adhesion and proliferation rate, and aligned nanofibers guided cell growth along the longitudinal axis of the nanofibers, which would provide a beneficial approach for tissue engineering. |
format | Online Article Text |
id | pubmed-4601863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-46018632015-10-13 Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite Razavi, Shahnaz Karbasi, Saeed Morshed, Mohammad Zarkesh Esfahani, Hamid Golozar, Mohammad Vaezifar, Sedigheh Cell J Original Article OBJECTIVE: In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffolds was then evaluated. MATERIALS AND METHODS: In this experimental study, the PLGA/CS scaffolds were prepared at the different ratios of 90/10, 80/20, and 70/30 (w/w) %. Morphology, cell adhesion and prolif- eration rate of h-ADSCs on the scaffolds were assessed using scanning electron microscope (SEM), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and trypan blue staining respectively. RESULTS: H-ADSCs seeded on the matrices indicated that the PLGA/CS composite matrix with aligned nanofibres and higher content of CS nano-powders gave significantly better performance than others in terms of cell adhesion and proliferation rate (P<0.05). CONCLUSION: We found that CS enhanced cell adhesion and proliferation rate, and aligned nanofibers guided cell growth along the longitudinal axis of the nanofibers, which would provide a beneficial approach for tissue engineering. Royan Institute 2015 2015-10-07 /pmc/articles/PMC4601863/ /pubmed/26464814 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Razavi, Shahnaz Karbasi, Saeed Morshed, Mohammad Zarkesh Esfahani, Hamid Golozar, Mohammad Vaezifar, Sedigheh Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite |
title | Cell Attachment and Proliferation of Human
Adipose-Derived Stem Cells on PLGA/Chitosan
Electrospun Nano-Biocomposite |
title_full | Cell Attachment and Proliferation of Human
Adipose-Derived Stem Cells on PLGA/Chitosan
Electrospun Nano-Biocomposite |
title_fullStr | Cell Attachment and Proliferation of Human
Adipose-Derived Stem Cells on PLGA/Chitosan
Electrospun Nano-Biocomposite |
title_full_unstemmed | Cell Attachment and Proliferation of Human
Adipose-Derived Stem Cells on PLGA/Chitosan
Electrospun Nano-Biocomposite |
title_short | Cell Attachment and Proliferation of Human
Adipose-Derived Stem Cells on PLGA/Chitosan
Electrospun Nano-Biocomposite |
title_sort | cell attachment and proliferation of human
adipose-derived stem cells on plga/chitosan
electrospun nano-biocomposite |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601863/ https://www.ncbi.nlm.nih.gov/pubmed/26464814 |
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