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Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite

OBJECTIVE: In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffold...

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Autores principales: Razavi, Shahnaz, Karbasi, Saeed, Morshed, Mohammad, Zarkesh Esfahani, Hamid, Golozar, Mohammad, Vaezifar, Sedigheh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601863/
https://www.ncbi.nlm.nih.gov/pubmed/26464814
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author Razavi, Shahnaz
Karbasi, Saeed
Morshed, Mohammad
Zarkesh Esfahani, Hamid
Golozar, Mohammad
Vaezifar, Sedigheh
author_facet Razavi, Shahnaz
Karbasi, Saeed
Morshed, Mohammad
Zarkesh Esfahani, Hamid
Golozar, Mohammad
Vaezifar, Sedigheh
author_sort Razavi, Shahnaz
collection PubMed
description OBJECTIVE: In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffolds was then evaluated. MATERIALS AND METHODS: In this experimental study, the PLGA/CS scaffolds were prepared at the different ratios of 90/10, 80/20, and 70/30 (w/w) %. Morphology, cell adhesion and prolif- eration rate of h-ADSCs on the scaffolds were assessed using scanning electron microscope (SEM), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and trypan blue staining respectively. RESULTS: H-ADSCs seeded on the matrices indicated that the PLGA/CS composite matrix with aligned nanofibres and higher content of CS nano-powders gave significantly better performance than others in terms of cell adhesion and proliferation rate (P<0.05). CONCLUSION: We found that CS enhanced cell adhesion and proliferation rate, and aligned nanofibers guided cell growth along the longitudinal axis of the nanofibers, which would provide a beneficial approach for tissue engineering.
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spelling pubmed-46018632015-10-13 Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite Razavi, Shahnaz Karbasi, Saeed Morshed, Mohammad Zarkesh Esfahani, Hamid Golozar, Mohammad Vaezifar, Sedigheh Cell J Original Article OBJECTIVE: In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffolds was then evaluated. MATERIALS AND METHODS: In this experimental study, the PLGA/CS scaffolds were prepared at the different ratios of 90/10, 80/20, and 70/30 (w/w) %. Morphology, cell adhesion and prolif- eration rate of h-ADSCs on the scaffolds were assessed using scanning electron microscope (SEM), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and trypan blue staining respectively. RESULTS: H-ADSCs seeded on the matrices indicated that the PLGA/CS composite matrix with aligned nanofibres and higher content of CS nano-powders gave significantly better performance than others in terms of cell adhesion and proliferation rate (P<0.05). CONCLUSION: We found that CS enhanced cell adhesion and proliferation rate, and aligned nanofibers guided cell growth along the longitudinal axis of the nanofibers, which would provide a beneficial approach for tissue engineering. Royan Institute 2015 2015-10-07 /pmc/articles/PMC4601863/ /pubmed/26464814 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Razavi, Shahnaz
Karbasi, Saeed
Morshed, Mohammad
Zarkesh Esfahani, Hamid
Golozar, Mohammad
Vaezifar, Sedigheh
Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite
title Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite
title_full Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite
title_fullStr Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite
title_full_unstemmed Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite
title_short Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite
title_sort cell attachment and proliferation of human adipose-derived stem cells on plga/chitosan electrospun nano-biocomposite
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601863/
https://www.ncbi.nlm.nih.gov/pubmed/26464814
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