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Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment

Hypersaline environments are considered one of the most extreme habitats on earth and microorganisms have developed diverse molecular mechanisms of adaptation to withstand these conditions. The present study was aimed at identifying novel genes from the microbial communities of a moderate-salinity r...

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Autores principales: Mirete, Salvador, Mora-Ruiz, Merit R., Lamprecht-Grandío, María, de Figueras, Carolina G., Rosselló-Móra, Ramon, González-Pastor, José E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602150/
https://www.ncbi.nlm.nih.gov/pubmed/26528268
http://dx.doi.org/10.3389/fmicb.2015.01121
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author Mirete, Salvador
Mora-Ruiz, Merit R.
Lamprecht-Grandío, María
de Figueras, Carolina G.
Rosselló-Móra, Ramon
González-Pastor, José E.
author_facet Mirete, Salvador
Mora-Ruiz, Merit R.
Lamprecht-Grandío, María
de Figueras, Carolina G.
Rosselló-Móra, Ramon
González-Pastor, José E.
author_sort Mirete, Salvador
collection PubMed
description Hypersaline environments are considered one of the most extreme habitats on earth and microorganisms have developed diverse molecular mechanisms of adaptation to withstand these conditions. The present study was aimed at identifying novel genes from the microbial communities of a moderate-salinity rhizosphere and brine from the Es Trenc saltern (Mallorca, Spain), which could confer increased salt resistance to Escherichia coli. The microbial diversity assessed by pyrosequencing of 16S rRNA gene libraries revealed the presence of communities that are typical in such environments and the remarkable presence of three bacterial groups never revealed as major components of salt brines. Metagenomic libraries from brine and rhizosphere samples, were transferred to the osmosensitive strain E. coli MKH13, and screened for salt resistance. Eleven genes that conferred salt resistance were identified, some encoding for well-known proteins previously related to osmoadaptation such as a glycerol transporter and a proton pump, whereas others encoded proteins not previously related to this function in microorganisms such as DNA/RNA helicases, an endonuclease III (Nth) and hypothetical proteins of unknown function. Furthermore, four of the retrieved genes were cloned and expressed in Bacillus subtilis and they also conferred salt resistance to this bacterium, broadening the spectrum of bacterial species in which these genes can function. This is the first report of salt resistance genes recovered from metagenomes of a hypersaline environment.
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spelling pubmed-46021502015-11-02 Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment Mirete, Salvador Mora-Ruiz, Merit R. Lamprecht-Grandío, María de Figueras, Carolina G. Rosselló-Móra, Ramon González-Pastor, José E. Front Microbiol Microbiology Hypersaline environments are considered one of the most extreme habitats on earth and microorganisms have developed diverse molecular mechanisms of adaptation to withstand these conditions. The present study was aimed at identifying novel genes from the microbial communities of a moderate-salinity rhizosphere and brine from the Es Trenc saltern (Mallorca, Spain), which could confer increased salt resistance to Escherichia coli. The microbial diversity assessed by pyrosequencing of 16S rRNA gene libraries revealed the presence of communities that are typical in such environments and the remarkable presence of three bacterial groups never revealed as major components of salt brines. Metagenomic libraries from brine and rhizosphere samples, were transferred to the osmosensitive strain E. coli MKH13, and screened for salt resistance. Eleven genes that conferred salt resistance were identified, some encoding for well-known proteins previously related to osmoadaptation such as a glycerol transporter and a proton pump, whereas others encoded proteins not previously related to this function in microorganisms such as DNA/RNA helicases, an endonuclease III (Nth) and hypothetical proteins of unknown function. Furthermore, four of the retrieved genes were cloned and expressed in Bacillus subtilis and they also conferred salt resistance to this bacterium, broadening the spectrum of bacterial species in which these genes can function. This is the first report of salt resistance genes recovered from metagenomes of a hypersaline environment. Frontiers Media S.A. 2015-10-13 /pmc/articles/PMC4602150/ /pubmed/26528268 http://dx.doi.org/10.3389/fmicb.2015.01121 Text en Copyright © 2015 Mirete, Mora-Ruiz, Lamprecht-Grandío, de Figueras, Rosselló-Móra and González-Pastor. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Mirete, Salvador
Mora-Ruiz, Merit R.
Lamprecht-Grandío, María
de Figueras, Carolina G.
Rosselló-Móra, Ramon
González-Pastor, José E.
Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment
title Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment
title_full Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment
title_fullStr Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment
title_full_unstemmed Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment
title_short Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment
title_sort salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602150/
https://www.ncbi.nlm.nih.gov/pubmed/26528268
http://dx.doi.org/10.3389/fmicb.2015.01121
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