Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis

Although there have been many recent advances in Tuberculosis (TB) detection technologies, there still remains a major need to develop simpler point-of-care techniques. In an effort towards such a diagnostic test for resource-poor settings, we have designed a bioassay based on detecting amplified DN...

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Detalles Bibliográficos
Autores principales: Ng, Benjamin Y.C., Wee, Eugene J.H., West, Nicholas P., Trau, Matt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602229/
http://dx.doi.org/10.1038/srep15027
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author Ng, Benjamin Y.C.
Wee, Eugene J.H.
West, Nicholas P.
Trau, Matt
author_facet Ng, Benjamin Y.C.
Wee, Eugene J.H.
West, Nicholas P.
Trau, Matt
author_sort Ng, Benjamin Y.C.
collection PubMed
description Although there have been many recent advances in Tuberculosis (TB) detection technologies, there still remains a major need to develop simpler point-of-care techniques. In an effort towards such a diagnostic test for resource-poor settings, we have designed a bioassay based on detecting amplified DNA via bridging flocculation. The assay is cheap, with a sensitivity approaching a single cell of Mycobacterium tuberculosis and the potential for translation into broader applications.
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spelling pubmed-46022292015-10-23 Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis Ng, Benjamin Y.C. Wee, Eugene J.H. West, Nicholas P. Trau, Matt Sci Rep Article Although there have been many recent advances in Tuberculosis (TB) detection technologies, there still remains a major need to develop simpler point-of-care techniques. In an effort towards such a diagnostic test for resource-poor settings, we have designed a bioassay based on detecting amplified DNA via bridging flocculation. The assay is cheap, with a sensitivity approaching a single cell of Mycobacterium tuberculosis and the potential for translation into broader applications. Nature Publishing Group 2015-10-13 /pmc/articles/PMC4602229/ http://dx.doi.org/10.1038/srep15027 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ng, Benjamin Y.C.
Wee, Eugene J.H.
West, Nicholas P.
Trau, Matt
Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis
title Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis
title_full Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis
title_fullStr Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis
title_full_unstemmed Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis
title_short Rapid DNA detection of Mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis
title_sort rapid dna detection of mycobacterium tuberculosis-towards single cell sensitivity in point-of-care diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602229/
http://dx.doi.org/10.1038/srep15027
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