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Connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer

OBJECTIVES: To evaluate the protein expression of connexin 43 (Cx43) in primary urothelial bladder cancer and test its association with the histopathological characteristics and clinical outcome. METHODS: A tissue microarray containing 348 tissue samples from 174 patients with primary urothelial car...

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Autores principales: Poyet, Cédric, Buser, Lorenz, Roudnicky, Filip, Detmar, Michael, Hermanns, Thomas, Mannhard, Doris, Höhn, Andrej, Rüschoff, Jan, Zhong, Qing, Sulser, Tullio, Moch, Holger, Wild, Peter J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602233/
https://www.ncbi.nlm.nih.gov/pubmed/26251520
http://dx.doi.org/10.1136/jclinpath-2015-202898
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author Poyet, Cédric
Buser, Lorenz
Roudnicky, Filip
Detmar, Michael
Hermanns, Thomas
Mannhard, Doris
Höhn, Andrej
Rüschoff, Jan
Zhong, Qing
Sulser, Tullio
Moch, Holger
Wild, Peter J
author_facet Poyet, Cédric
Buser, Lorenz
Roudnicky, Filip
Detmar, Michael
Hermanns, Thomas
Mannhard, Doris
Höhn, Andrej
Rüschoff, Jan
Zhong, Qing
Sulser, Tullio
Moch, Holger
Wild, Peter J
author_sort Poyet, Cédric
collection PubMed
description OBJECTIVES: To evaluate the protein expression of connexin 43 (Cx43) in primary urothelial bladder cancer and test its association with the histopathological characteristics and clinical outcome. METHODS: A tissue microarray containing 348 tissue samples from 174 patients with primary urothelial carcinomas of the bladder was immunohistochemically stained for Cx43. The intensity of staining was semiquantitatively evaluated (score 0, 1+, 2+), and the association with clinicopathological features was assessed. Univariable and multivariable analyses were performed to identify predictors for progression-free survival (PFS). RESULTS: Membranous Cx43 immunoreactivity was detected in 118 (67.8%) of 174 analysable urothelial carcinomas, of which 31 (17.8%) showed even a strong (score 2+) and mainly homogeneous staining. Strong expression levels of Cx43 (score 2+) were associated with higher tumour grade, multiplicity and increased proliferation (all p<0.05). In the subgroup of patients with stage pTa and pT1 bladder tumours (n=158), strong Cx43 expression (p<0.001), solid growth pattern (p<0.001) and increased Ki-67 proliferation fraction (p<0.05) were significantly associated with shorter PFS in an univariable Cox regression analysis. In multivariable Cox regression models, Cx43 immunoreactivity and histological growth pattern remained highly significant and adverse risk factors for PFS. CONCLUSIONS: The expression levels of Cx43 are frequent in non-muscle invasive bladder cancer (NMIBC), with high expression levels being associated with poor prognosis. Routine assessment of Cx43 expression may improve the identification of high-risk NMIBC.
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spelling pubmed-46022332015-10-21 Connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer Poyet, Cédric Buser, Lorenz Roudnicky, Filip Detmar, Michael Hermanns, Thomas Mannhard, Doris Höhn, Andrej Rüschoff, Jan Zhong, Qing Sulser, Tullio Moch, Holger Wild, Peter J J Clin Pathol Original Article OBJECTIVES: To evaluate the protein expression of connexin 43 (Cx43) in primary urothelial bladder cancer and test its association with the histopathological characteristics and clinical outcome. METHODS: A tissue microarray containing 348 tissue samples from 174 patients with primary urothelial carcinomas of the bladder was immunohistochemically stained for Cx43. The intensity of staining was semiquantitatively evaluated (score 0, 1+, 2+), and the association with clinicopathological features was assessed. Univariable and multivariable analyses were performed to identify predictors for progression-free survival (PFS). RESULTS: Membranous Cx43 immunoreactivity was detected in 118 (67.8%) of 174 analysable urothelial carcinomas, of which 31 (17.8%) showed even a strong (score 2+) and mainly homogeneous staining. Strong expression levels of Cx43 (score 2+) were associated with higher tumour grade, multiplicity and increased proliferation (all p<0.05). In the subgroup of patients with stage pTa and pT1 bladder tumours (n=158), strong Cx43 expression (p<0.001), solid growth pattern (p<0.001) and increased Ki-67 proliferation fraction (p<0.05) were significantly associated with shorter PFS in an univariable Cox regression analysis. In multivariable Cox regression models, Cx43 immunoreactivity and histological growth pattern remained highly significant and adverse risk factors for PFS. CONCLUSIONS: The expression levels of Cx43 are frequent in non-muscle invasive bladder cancer (NMIBC), with high expression levels being associated with poor prognosis. Routine assessment of Cx43 expression may improve the identification of high-risk NMIBC. BMJ Publishing Group 2015-10 2015-08-06 /pmc/articles/PMC4602233/ /pubmed/26251520 http://dx.doi.org/10.1136/jclinpath-2015-202898 Text en Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Original Article
Poyet, Cédric
Buser, Lorenz
Roudnicky, Filip
Detmar, Michael
Hermanns, Thomas
Mannhard, Doris
Höhn, Andrej
Rüschoff, Jan
Zhong, Qing
Sulser, Tullio
Moch, Holger
Wild, Peter J
Connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer
title Connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer
title_full Connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer
title_fullStr Connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer
title_full_unstemmed Connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer
title_short Connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer
title_sort connexin 43 expression predicts poor progression-free survival in patients with non-muscle invasive urothelial bladder cancer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602233/
https://www.ncbi.nlm.nih.gov/pubmed/26251520
http://dx.doi.org/10.1136/jclinpath-2015-202898
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