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Comparative evaluation of commercially available manual and automated nucleic acid extraction methods for rotavirus RNA detection in stools
Rotaviruses are a major cause of viral gastroenteritis in children. For accurate and sensitive detection of rotavirus RNA from stool samples by reverse transcription-polymerase chain reaction (RT-PCR), the extraction process must be robust. However, some extraction methods may not remove the strong...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier/North-Holland Biomedical Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603280/ https://www.ncbi.nlm.nih.gov/pubmed/24036075 http://dx.doi.org/10.1016/j.jviromet.2013.08.023 |