Cargando…
Comparative evaluation of commercially available manual and automated nucleic acid extraction methods for rotavirus RNA detection in stools
Rotaviruses are a major cause of viral gastroenteritis in children. For accurate and sensitive detection of rotavirus RNA from stool samples by reverse transcription-polymerase chain reaction (RT-PCR), the extraction process must be robust. However, some extraction methods may not remove the strong...
Autores principales: | Esona, Mathew D., McDonald, Sharla, Kamili, Shifaq, Kerin, Tara, Gautam, Rashi, Bowen, Michael D. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier/North-Holland Biomedical Press
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603280/ https://www.ncbi.nlm.nih.gov/pubmed/24036075 http://dx.doi.org/10.1016/j.jviromet.2013.08.023 |
Ejemplares similares
-
Real-time RT-PCR assays to differentiate wild-type group A rotavirus strains from Rotarix(®) and RotaTeq(®) vaccine strains in stool samples
por: Gautam, Rashi, et al.
Publicado: (2014) -
One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples
por: Gautam, Rashi, et al.
Publicado: (2016) -
Molecular characteristics of rotavirus genotypes circulating in the south of Benin, 2016–2018
por: Agbla, Jijoho Michel, et al.
Publicado: (2020) -
Whole gene analysis of a genotype G29P[6] human rotavirus strain identified in Central African Republic
por: Banga-Mingo, Virginie, et al.
Publicado: (2021) -
Detection of diarrhoea associated rotavirus and co-infection with diarrhoeagenic pathogens in the Littoral region of Cameroon using ELISA, RT-PCR and Luminex xTAG GPP assays
por: Ghapoutsa, Rahinatou N., et al.
Publicado: (2021)