First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation

Introduction Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TE...

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Autores principales: Lukaszuk, Krzysztof, Wcislo, Monika, Liss, Joanna, Stachowicz, Anna, Jakiel, Grzegorz, Lukaszuk, Mariusz, Pastuszek, Ewa, Woclawek-Potocka, Izabela, Galvao, Antonio, Bialobrzeska, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Medical Publishers 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603856/
https://www.ncbi.nlm.nih.gov/pubmed/26495164
http://dx.doi.org/10.1055/s-0035-1548726
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author Lukaszuk, Krzysztof
Wcislo, Monika
Liss, Joanna
Stachowicz, Anna
Jakiel, Grzegorz
Lukaszuk, Mariusz
Pastuszek, Ewa
Woclawek-Potocka, Izabela
Galvao, Antonio
Bialobrzeska, Dorota
author_facet Lukaszuk, Krzysztof
Wcislo, Monika
Liss, Joanna
Stachowicz, Anna
Jakiel, Grzegorz
Lukaszuk, Mariusz
Pastuszek, Ewa
Woclawek-Potocka, Izabela
Galvao, Antonio
Bialobrzeska, Dorota
author_sort Lukaszuk, Krzysztof
collection PubMed
description Introduction Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used. Conclusion The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done.
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spelling pubmed-46038562015-10-22 First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation Lukaszuk, Krzysztof Wcislo, Monika Liss, Joanna Stachowicz, Anna Jakiel, Grzegorz Lukaszuk, Mariusz Pastuszek, Ewa Woclawek-Potocka, Izabela Galvao, Antonio Bialobrzeska, Dorota AJP Rep Article Introduction Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used. Conclusion The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done. Thieme Medical Publishers 2015-04-06 2015-10 /pmc/articles/PMC4603856/ /pubmed/26495164 http://dx.doi.org/10.1055/s-0035-1548726 Text en © Thieme Medical Publishers
spellingShingle Article
Lukaszuk, Krzysztof
Wcislo, Monika
Liss, Joanna
Stachowicz, Anna
Jakiel, Grzegorz
Lukaszuk, Mariusz
Pastuszek, Ewa
Woclawek-Potocka, Izabela
Galvao, Antonio
Bialobrzeska, Dorota
First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
title First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
title_full First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
title_fullStr First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
title_full_unstemmed First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
title_short First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation
title_sort first pregnancy, somatic and psychological status of a 4-year-old child born following annexin v tesa sperm separation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603856/
https://www.ncbi.nlm.nih.gov/pubmed/26495164
http://dx.doi.org/10.1055/s-0035-1548726
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