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The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification
OBJECTIVE: The goal of this study was to ascertain optimal assisted hatching (AH) method in frozen embryo transfer. We compared the effect of depending on whether mechanical or laser-AH was performed before or after the vitrification of embryo development rate and blastocyst cell numbers. METHODS: I...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Reproductive Medicine
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604299/ https://www.ncbi.nlm.nih.gov/pubmed/26473108 http://dx.doi.org/10.5653/cerm.2015.42.3.94 |
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author | Kim, Hye Jin Lee, Ki Hwan Park, Sung Baek Choi, Young Bae Yang, Jung Bo |
author_facet | Kim, Hye Jin Lee, Ki Hwan Park, Sung Baek Choi, Young Bae Yang, Jung Bo |
author_sort | Kim, Hye Jin |
collection | PubMed |
description | OBJECTIVE: The goal of this study was to ascertain optimal assisted hatching (AH) method in frozen embryo transfer. We compared the effect of depending on whether mechanical or laser-AH was performed before or after the vitrification of embryo development rate and blastocyst cell numbers. METHODS: In order to induce superovulation, pregnant mare's serum gonadotropin followed by human chorionic gonadotropin were injected into 4- to 5-week-old female mice. 2-cell embryos were then collected by flushing out the oviducts. The Expanded blastocysts were recovered after the collected embryos were incubated for 48 hours, and were then subjected to artificial shrinkage (AS) and cross-mechanical AH (cMAH) or quarter-laser zona thinning-AH (qLZT-AH) were carried out using the expanded blastocysts before or after vitrification. After 48 hours of incubation, followed by vitrification and thawing (V-T), and blastocysts were fluorescence stained and observed. RESULTS: The rate of formation of hatched blastocysts after 24 and 72 hours of incubation was significantly higher in the AS/qLZT-AH/V-T group than in the other groups (p<0.05). The cell number of the inner cell mass was higher in AS/V-T/non-AH and AS/V-T/cMAH groups than those of others (p<0.05). In the control group, the number of trophectoderm and the total cell number were higher than in the AS-AH group (p<0.05). CONCLUSION: The above results suggest that AS and AH in vitrification of expanded blastocysts lead to the more efficient formation of hatched blastocysts in mice. |
format | Online Article Text |
id | pubmed-4604299 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Korean Society for Reproductive Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-46042992015-10-15 The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification Kim, Hye Jin Lee, Ki Hwan Park, Sung Baek Choi, Young Bae Yang, Jung Bo Clin Exp Reprod Med Original Article OBJECTIVE: The goal of this study was to ascertain optimal assisted hatching (AH) method in frozen embryo transfer. We compared the effect of depending on whether mechanical or laser-AH was performed before or after the vitrification of embryo development rate and blastocyst cell numbers. METHODS: In order to induce superovulation, pregnant mare's serum gonadotropin followed by human chorionic gonadotropin were injected into 4- to 5-week-old female mice. 2-cell embryos were then collected by flushing out the oviducts. The Expanded blastocysts were recovered after the collected embryos were incubated for 48 hours, and were then subjected to artificial shrinkage (AS) and cross-mechanical AH (cMAH) or quarter-laser zona thinning-AH (qLZT-AH) were carried out using the expanded blastocysts before or after vitrification. After 48 hours of incubation, followed by vitrification and thawing (V-T), and blastocysts were fluorescence stained and observed. RESULTS: The rate of formation of hatched blastocysts after 24 and 72 hours of incubation was significantly higher in the AS/qLZT-AH/V-T group than in the other groups (p<0.05). The cell number of the inner cell mass was higher in AS/V-T/non-AH and AS/V-T/cMAH groups than those of others (p<0.05). In the control group, the number of trophectoderm and the total cell number were higher than in the AS-AH group (p<0.05). CONCLUSION: The above results suggest that AS and AH in vitrification of expanded blastocysts lead to the more efficient formation of hatched blastocysts in mice. The Korean Society for Reproductive Medicine 2015-09 2015-09-30 /pmc/articles/PMC4604299/ /pubmed/26473108 http://dx.doi.org/10.5653/cerm.2015.42.3.94 Text en Copyright © 2015. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Hye Jin Lee, Ki Hwan Park, Sung Baek Choi, Young Bae Yang, Jung Bo The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification |
title | The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification |
title_full | The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification |
title_fullStr | The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification |
title_full_unstemmed | The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification |
title_short | The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification |
title_sort | effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604299/ https://www.ncbi.nlm.nih.gov/pubmed/26473108 http://dx.doi.org/10.5653/cerm.2015.42.3.94 |
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