A mass spectrometry-based method for comprehensive quantitative determination of post-transcriptional RNA modifications: the complete chemical structure of Schizosaccharomyces pombe ribosomal RNAs

We present a liquid chromatography–mass spectrometry (LC-MS)-based method for comprehensive quantitative identification of post-transcriptional modifications (PTMs) of RNA. We incorporated an in vitro-transcribed, heavy isotope-labeled reference RNA into a sample RNA solution, digested the mixture w...

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Detalles Bibliográficos
Autores principales: Taoka, Masato, Nobe, Yuko, Hori, Masayuki, Takeuchi, Aiko, Masaki, Shunpei, Yamauchi, Yoshio, Nakayama, Hiroshi, Takahashi, Nobuhiro, Isobe, Toshiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605285/
https://www.ncbi.nlm.nih.gov/pubmed/26013808
http://dx.doi.org/10.1093/nar/gkv560

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