Cargando…

Bacteriophage strain typing by rapid single molecule analysis

Rapid characterization of unknown biological samples is under the focus of many current studies. Here we report a method for screening of biological samples by optical mapping of their DNA. We use a novel, one-step chemo-enzymatic reaction to covalently bind fluorophores to DNA at the four-base reco...

Descripción completa

Detalles Bibliográficos
Autores principales:	Grunwald, Assaf, Dahan, Moran, Giesbertz, Anna, Nilsson, Adam, Nyberg, Lena K., Weinhold, Elmar, Ambjörnsson, Tobias, Westerlund, Fredrik, Ebenstein, Yuval
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605287/ https://www.ncbi.nlm.nih.gov/pubmed/26019180 http://dx.doi.org/10.1093/nar/gkv563

Ejemplares similares

Enzyme-free optical DNA mapping of the human genome using competitive binding
por: Müller, Vilhelm, et al.
Publicado: (2019)

Competitive binding-based optical DNA mapping for fast identification of bacteria - multi-ligand transfer matrix theory and experimental applications on Escherichia coli
por: Nilsson, Adam N., et al.
Publicado: (2014)

Heterogeneous staining: a tool for studies of how fluorescent dyes affect the physical properties of DNA
por: Nyberg, Lena, et al.
Publicado: (2013)

Chemoenzymatic labeling of DNA methylation patterns for single-molecule epigenetic mapping
por: Gabrieli, Tslil, et al.
Publicado: (2022)

Sizing femtogram amounts of dsDNA by single-molecule counting
por: Torchinsky, Dmitry, et al.
Publicado: (2016)

Long-read single-molecule maps of the functional methylome
por: Sharim, Hila, et al.
Publicado: (2019)

Selective nanopore sequencing of human BRCA1 by Cas9-assisted targeting of chromosome segments (CATCH)
por: Gabrieli, Tslil, et al.
Publicado: (2018)

MLGA—a rapid and cost-efficient assay for gene copy-number analysis
por: Isaksson, Magnus, et al.
Publicado: (2007)

Rapid DNA mapping by fluorescent single molecule detection
por: Xiao, Ming, et al.
Publicado: (2007)

DNA barcodes for rapid, whole genome, single-molecule analyses
por: Wand, Nathaniel O, et al.
Publicado: (2019)

Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules
por: Nyberg, Lena K., et al.
Publicado: (2016)

Bacteriophage Mu integration in yeast and mammalian genomes
por: Paatero, Anja O., et al.
Publicado: (2008)

Rapid single-molecule characterisation of enzymes involved in nucleic-acid metabolism
por: Mueller, Stefan H, et al.
Publicado: (2022)

Corrigendum: Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules
por: Nyberg, Lena K., et al.
Publicado: (2017)

Disturbance-free rapid solution exchange for magnetic tweezers single-molecule studies
por: Le, Shimin, et al.
Publicado: (2015)

Single molecule analysis of combinatorial splicing
por: Conze, Tim, et al.
Publicado: (2010)

Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging
por: Gunnarsson, Anders, et al.
Publicado: (2009)

A simple method for displaying recalcitrant proteins on the surface of bacteriophage lambda
por: Zanghi, Christine N., et al.
Publicado: (2005)

Simultaneous amplification and screening of whole plasmids using the T7 bacteriophage replisome
por: Xu, Yan, et al.
Publicado: (2006)

Selection of bacteriophage λ integrases with altered recombination specificity by in vitro compartmentalization
por: Tay, Yvonne, et al.
Publicado: (2010)

A single molecule array for digital targeted molecular analyses
por: Göransson, Jenny, et al.
Publicado: (2009)

Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping
por: Müller, Vilhelm, et al.
Publicado: (2016)

Sensitive and inexpensive digital DNA analysis by microfluidic enrichment of rolling circle amplified single-molecules
por: Kühnemund, Malte, et al.
Publicado: (2017)

PieceMaker: selection of DNA fragments for selector-guided multiplex amplification
por: Stenberg, Johan, et al.
Publicado: (2005)

AssemblX: a user-friendly toolkit for rapid and reliable multi-gene assemblies
por: Hochrein, Lena, et al.
Publicado: (2017)

Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
por: Dahl, Fredrik, et al.
Publicado: (2005)

A tractable method for simultaneous modifications to the head and tail of bacteriophage lambda and its application to enhancing phage-mediated gene delivery
por: Zanghi, Christine N., et al.
Publicado: (2007)

Immobilized stem–loop structured probes as conformational switches for enzymatic detection of microbial 16S rRNA
por: Bockisch, Benjamin, et al.
Publicado: (2005)

Rapid detection of genomic imbalances using micro-arrays consisting of pooled BACs covering all human chromosome arms
por: Knijnenburg, Jeroen, et al.
Publicado: (2005)

Identification of antisense nucleic acid hybridization sites in mRNA molecules with self-quenching fluorescent reporter molecules
por: Gifford, Lida K., et al.
Publicado: (2005)

Single-molecule analysis of genome rearrangements in cancer
por: Pole, Jessica C. M., et al.
Publicado: (2011)

Single molecule mass photometry of nucleic acids
por: Li, Yiwen, et al.
Publicado: (2020)

Single-molecule detection of deoxyribonucleoside triphosphates in microdroplets
por: Breiner, Boris, et al.
Publicado: (2019)

Evolution-guided engineering of small-molecule biosensors
por: Snoek, Tim, et al.
Publicado: (2020)

Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform
por: Wagner, Tobias, et al.
Publicado: (2016)

Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules
por: Cebrián, Jorge, et al.
Publicado: (2015)

Mapping DNA polymerase errors by single-molecule sequencing
por: Lee, David F., et al.
Publicado: (2016)

TAMRA-polypyrrole for A/T sequence visualization on DNA molecules
por: Lee, Seonghyun, et al.
Publicado: (2018)

Fluorescence spectroscopic detection and measurement of single telomere molecules
por: Beh, Cyrus W, et al.
Publicado: (2018)

Summarizing and correcting the GC content bias in high-throughput sequencing
por: Benjamini, Yuval, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_prbb4jk61k3evq9lr6ra1l6c22