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Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis

Interleukin-10 overproduction has been associated with worse prognosis in human cutaneous leishmaniasis, while IFN-γ-dependent responses are associated with parasite killing and host protection. Innovative strategies are needed to overcome therapeutic failure observed in endemic areas. The use of mo...

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Autores principales: Castellano, Lucio Roberto, Argiro, Laurent, Dessein, Helia, Dessein, Alain, da Silva, Marcos Vinícius, Correia, Dalmo, Rodrigues, Virmondes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606169/
https://www.ncbi.nlm.nih.gov/pubmed/26495321
http://dx.doi.org/10.1155/2015/152741
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author Castellano, Lucio Roberto
Argiro, Laurent
Dessein, Helia
Dessein, Alain
da Silva, Marcos Vinícius
Correia, Dalmo
Rodrigues, Virmondes
author_facet Castellano, Lucio Roberto
Argiro, Laurent
Dessein, Helia
Dessein, Alain
da Silva, Marcos Vinícius
Correia, Dalmo
Rodrigues, Virmondes
author_sort Castellano, Lucio Roberto
collection PubMed
description Interleukin-10 overproduction has been associated with worse prognosis in human cutaneous leishmaniasis, while IFN-γ-dependent responses are associated with parasite killing and host protection. Innovative strategies are needed to overcome therapeutic failure observed in endemic areas. The use of monoclonal antibody-based immunotherapy targeting IL-10 cytokine was evaluated here. Partial IL-10 blockade in Leishmania braziliensis whole soluble antigen-stimulated cells from endemic area CL patients with active or healed lesions and asymptomatic controls was evaluated. Overall decrease in IL-10, IL-4, and TNF-α production was observed in all groups of subjects. Only patients with active lesions still produced some levels of TNF-α after anti-IL-10 stimulation in association with Leishmania antigens. Moreover, this strategy showed limited modulatory effects on IFN-γ-dependent chemokine CXCL10 production. Results suggest the potential immunotherapeutic use of partial IL-10 blockade in localized cutaneous leishmaniasis.
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spelling pubmed-46061692015-10-22 Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis Castellano, Lucio Roberto Argiro, Laurent Dessein, Helia Dessein, Alain da Silva, Marcos Vinícius Correia, Dalmo Rodrigues, Virmondes J Immunol Res Research Article Interleukin-10 overproduction has been associated with worse prognosis in human cutaneous leishmaniasis, while IFN-γ-dependent responses are associated with parasite killing and host protection. Innovative strategies are needed to overcome therapeutic failure observed in endemic areas. The use of monoclonal antibody-based immunotherapy targeting IL-10 cytokine was evaluated here. Partial IL-10 blockade in Leishmania braziliensis whole soluble antigen-stimulated cells from endemic area CL patients with active or healed lesions and asymptomatic controls was evaluated. Overall decrease in IL-10, IL-4, and TNF-α production was observed in all groups of subjects. Only patients with active lesions still produced some levels of TNF-α after anti-IL-10 stimulation in association with Leishmania antigens. Moreover, this strategy showed limited modulatory effects on IFN-γ-dependent chemokine CXCL10 production. Results suggest the potential immunotherapeutic use of partial IL-10 blockade in localized cutaneous leishmaniasis. Hindawi Publishing Corporation 2015 2015-10-01 /pmc/articles/PMC4606169/ /pubmed/26495321 http://dx.doi.org/10.1155/2015/152741 Text en Copyright © 2015 Lucio Roberto Castellano et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Castellano, Lucio Roberto
Argiro, Laurent
Dessein, Helia
Dessein, Alain
da Silva, Marcos Vinícius
Correia, Dalmo
Rodrigues, Virmondes
Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis
title Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis
title_full Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis
title_fullStr Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis
title_full_unstemmed Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis
title_short Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis
title_sort potential use of interleukin-10 blockade as a therapeutic strategy in human cutaneous leishmaniasis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606169/
https://www.ncbi.nlm.nih.gov/pubmed/26495321
http://dx.doi.org/10.1155/2015/152741
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