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Enzymatic analysis of venom from Cuban scorpion Rhopalurus junceus

Rhopalurus junceus scorpion venom has been identified as a natural extract with anticancer potential. Interestingly, this scorpion venom does not cause adverse symptoms in humans. However, there is scarce information about its composition and enzymatic activity. In this work, we determined the elect...

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Autores principales: Díaz-García, Alexis, Ruiz-Fuentes, Jenny Laura, Yglesias-Rivera, Arianna, Rodríguez-Sánchez, Hermis, Riquenes Garlobo, Yanelis, Fleitas Martinez, Osmel, Fraga Castro, José A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Library Publishing Media 2015
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606921/
https://www.ncbi.nlm.nih.gov/pubmed/26605039
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author Díaz-García, Alexis
Ruiz-Fuentes, Jenny Laura
Yglesias-Rivera, Arianna
Rodríguez-Sánchez, Hermis
Riquenes Garlobo, Yanelis
Fleitas Martinez, Osmel
Fraga Castro, José A
author_facet Díaz-García, Alexis
Ruiz-Fuentes, Jenny Laura
Yglesias-Rivera, Arianna
Rodríguez-Sánchez, Hermis
Riquenes Garlobo, Yanelis
Fleitas Martinez, Osmel
Fraga Castro, José A
author_sort Díaz-García, Alexis
collection PubMed
description Rhopalurus junceus scorpion venom has been identified as a natural extract with anticancer potential. Interestingly, this scorpion venom does not cause adverse symptoms in humans. However, there is scarce information about its composition and enzymatic activity. In this work, we determined the electrophoretic profile of the venom, the gelatinase and caseinolytic activity, and the phospholipase A(2) (PLA(2)) and hemolytic activity. The effect of different venom doses (6.25, 12.5 and 25 mg/kg) on gastrocnemius muscle was also measured as CK and LDH activity in serum. The presence of hyaluronidase was determined by turbidimetric assay. The effect of different fractions obtained by gel filtration chromatography were evaluated at different concentrations (0.05, 0.1, 0.2, 0.4, 0.6mg/ml) against lung cancer cell A549 and lung normal cell MRC-5 using MTT assay. The electrophoretic profile demonstrated the presence of proteins bands around 67kDa, 43kDa, 18.4kDa and a majority band below 14.3kDa. The venom did not showed caseinolytic, gelatinase, PLA(2) and hemolytic activity even at highest venom concentration used in the study. Scorpion venom only showed a significant toxic effect on gastrocnemius muscles identified by CK and LDH release after subcutaneous injection of 12.5 and 25mg/kg. Low molecular weight fractions (<4kDa) induced a significant cytotoxicity in A549 cells while high molecular weight proteins (45–60kDa) were responsible for hyaluronidase activity and toxic effect against MRC-5. Experiments indicate that Rhopalurus junceus scorpion venom has low enzymatic activity, which could contribute to the low toxic potential of this scorpion venom.
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spelling pubmed-46069212015-11-24 Enzymatic analysis of venom from Cuban scorpion Rhopalurus junceus Díaz-García, Alexis Ruiz-Fuentes, Jenny Laura Yglesias-Rivera, Arianna Rodríguez-Sánchez, Hermis Riquenes Garlobo, Yanelis Fleitas Martinez, Osmel Fraga Castro, José A J Venom Res Research Article Rhopalurus junceus scorpion venom has been identified as a natural extract with anticancer potential. Interestingly, this scorpion venom does not cause adverse symptoms in humans. However, there is scarce information about its composition and enzymatic activity. In this work, we determined the electrophoretic profile of the venom, the gelatinase and caseinolytic activity, and the phospholipase A(2) (PLA(2)) and hemolytic activity. The effect of different venom doses (6.25, 12.5 and 25 mg/kg) on gastrocnemius muscle was also measured as CK and LDH activity in serum. The presence of hyaluronidase was determined by turbidimetric assay. The effect of different fractions obtained by gel filtration chromatography were evaluated at different concentrations (0.05, 0.1, 0.2, 0.4, 0.6mg/ml) against lung cancer cell A549 and lung normal cell MRC-5 using MTT assay. The electrophoretic profile demonstrated the presence of proteins bands around 67kDa, 43kDa, 18.4kDa and a majority band below 14.3kDa. The venom did not showed caseinolytic, gelatinase, PLA(2) and hemolytic activity even at highest venom concentration used in the study. Scorpion venom only showed a significant toxic effect on gastrocnemius muscles identified by CK and LDH release after subcutaneous injection of 12.5 and 25mg/kg. Low molecular weight fractions (<4kDa) induced a significant cytotoxicity in A549 cells while high molecular weight proteins (45–60kDa) were responsible for hyaluronidase activity and toxic effect against MRC-5. Experiments indicate that Rhopalurus junceus scorpion venom has low enzymatic activity, which could contribute to the low toxic potential of this scorpion venom. Library Publishing Media 2015-07-22 /pmc/articles/PMC4606921/ /pubmed/26605039 Text en © Copyright The Author(s) http://creativecommons.org/licenses/by-nc/3.0 First Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.
spellingShingle Research Article
Díaz-García, Alexis
Ruiz-Fuentes, Jenny Laura
Yglesias-Rivera, Arianna
Rodríguez-Sánchez, Hermis
Riquenes Garlobo, Yanelis
Fleitas Martinez, Osmel
Fraga Castro, José A
Enzymatic analysis of venom from Cuban scorpion Rhopalurus junceus
title Enzymatic analysis of venom from Cuban scorpion Rhopalurus junceus
title_full Enzymatic analysis of venom from Cuban scorpion Rhopalurus junceus
title_fullStr Enzymatic analysis of venom from Cuban scorpion Rhopalurus junceus
title_full_unstemmed Enzymatic analysis of venom from Cuban scorpion Rhopalurus junceus
title_short Enzymatic analysis of venom from Cuban scorpion Rhopalurus junceus
title_sort enzymatic analysis of venom from cuban scorpion rhopalurus junceus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606921/
https://www.ncbi.nlm.nih.gov/pubmed/26605039
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