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A Novel Dual Expression Platform for High Throughput Functional Screening of Phage Libraries in Product like Format
High throughput screenings of single chain Fv (scFv) antibody phage display libraries are currently done as soluble scFvs produced in E.coli. Due to endotoxin contaminations from bacterial cells these preparations cannot be reliably used in mammalian cell based assays. The monovalent nature and lack...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607404/ https://www.ncbi.nlm.nih.gov/pubmed/26468955 http://dx.doi.org/10.1371/journal.pone.0140691 |