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Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae

mRNA splicing is required in about 4% of protein coding genes in Saccharomyces cerevisiae. The gene structure of those genes is simple, generally comprising two exons and one intron. In order to characterize the impact of alternative splicing on the S. cerevisiae transcriptome, we perform a systemat...

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Detalles Bibliográficos
Autores principales: Schreiber, Konrad, Csaba, Gergely, Haslbeck, Martin, Zimmer, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607428/
https://www.ncbi.nlm.nih.gov/pubmed/26469855
http://dx.doi.org/10.1371/journal.pone.0140487
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author Schreiber, Konrad
Csaba, Gergely
Haslbeck, Martin
Zimmer, Ralf
author_facet Schreiber, Konrad
Csaba, Gergely
Haslbeck, Martin
Zimmer, Ralf
author_sort Schreiber, Konrad
collection PubMed
description mRNA splicing is required in about 4% of protein coding genes in Saccharomyces cerevisiae. The gene structure of those genes is simple, generally comprising two exons and one intron. In order to characterize the impact of alternative splicing on the S. cerevisiae transcriptome, we perform a systematic analysis of mRNA sequencing data. We find evidence of a pervasive use of alternative splice sites and detect several novel introns both within and outside protein coding regions. We also find a predominance of alternative splicing on the 3’ side of introns, a finding which is consistent with existing knowledge on conservation of exon-intron boundaries in S. cerevisiae. Some of the alternatively spliced transcripts allow for a translation into different protein products.
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spelling pubmed-46074282015-10-29 Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae Schreiber, Konrad Csaba, Gergely Haslbeck, Martin Zimmer, Ralf PLoS One Research Article mRNA splicing is required in about 4% of protein coding genes in Saccharomyces cerevisiae. The gene structure of those genes is simple, generally comprising two exons and one intron. In order to characterize the impact of alternative splicing on the S. cerevisiae transcriptome, we perform a systematic analysis of mRNA sequencing data. We find evidence of a pervasive use of alternative splice sites and detect several novel introns both within and outside protein coding regions. We also find a predominance of alternative splicing on the 3’ side of introns, a finding which is consistent with existing knowledge on conservation of exon-intron boundaries in S. cerevisiae. Some of the alternatively spliced transcripts allow for a translation into different protein products. Public Library of Science 2015-10-15 /pmc/articles/PMC4607428/ /pubmed/26469855 http://dx.doi.org/10.1371/journal.pone.0140487 Text en © 2015 Schreiber et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Schreiber, Konrad
Csaba, Gergely
Haslbeck, Martin
Zimmer, Ralf
Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae
title Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae
title_full Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae
title_fullStr Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae
title_full_unstemmed Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae
title_short Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae
title_sort alternative splicing in next generation sequencing data of saccharomyces cerevisiae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607428/
https://www.ncbi.nlm.nih.gov/pubmed/26469855
http://dx.doi.org/10.1371/journal.pone.0140487
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