Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding

New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectromet...

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Detalles Bibliográficos
Autores principales: Kirillova, Yuliya, Boyarskaya, Nataliya, Dezhenkov, Andrey, Tankevich, Mariya, Prokhorov, Ivan, Varizhuk, Anna, Eremin, Sergei, Esipov, Dmitry, Smirnov, Igor, Pozmogova, Galina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607454/
https://www.ncbi.nlm.nih.gov/pubmed/26469337
http://dx.doi.org/10.1371/journal.pone.0140468
Descripción
Sumario:New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T(10) PNA forms a stable triplex with polyadenine DNA.

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