Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding

New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectromet...

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Autores principales: Kirillova, Yuliya, Boyarskaya, Nataliya, Dezhenkov, Andrey, Tankevich, Mariya, Prokhorov, Ivan, Varizhuk, Anna, Eremin, Sergei, Esipov, Dmitry, Smirnov, Igor, Pozmogova, Galina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607454/
https://www.ncbi.nlm.nih.gov/pubmed/26469337
http://dx.doi.org/10.1371/journal.pone.0140468
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author Kirillova, Yuliya
Boyarskaya, Nataliya
Dezhenkov, Andrey
Tankevich, Mariya
Prokhorov, Ivan
Varizhuk, Anna
Eremin, Sergei
Esipov, Dmitry
Smirnov, Igor
Pozmogova, Galina
author_facet Kirillova, Yuliya
Boyarskaya, Nataliya
Dezhenkov, Andrey
Tankevich, Mariya
Prokhorov, Ivan
Varizhuk, Anna
Eremin, Sergei
Esipov, Dmitry
Smirnov, Igor
Pozmogova, Galina
author_sort Kirillova, Yuliya
collection PubMed
description New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T(10) PNA forms a stable triplex with polyadenine DNA.
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spelling pubmed-46074542015-10-29 Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding Kirillova, Yuliya Boyarskaya, Nataliya Dezhenkov, Andrey Tankevich, Mariya Prokhorov, Ivan Varizhuk, Anna Eremin, Sergei Esipov, Dmitry Smirnov, Igor Pozmogova, Galina PLoS One Research Article New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T(10) PNA forms a stable triplex with polyadenine DNA. Public Library of Science 2015-10-15 /pmc/articles/PMC4607454/ /pubmed/26469337 http://dx.doi.org/10.1371/journal.pone.0140468 Text en © 2015 Kirillova et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kirillova, Yuliya
Boyarskaya, Nataliya
Dezhenkov, Andrey
Tankevich, Mariya
Prokhorov, Ivan
Varizhuk, Anna
Eremin, Sergei
Esipov, Dmitry
Smirnov, Igor
Pozmogova, Galina
Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding
title Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding
title_full Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding
title_fullStr Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding
title_full_unstemmed Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding
title_short Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding
title_sort polyanionic carboxyethyl peptide nucleic acids (ce-pnas): synthesis and dna binding
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607454/
https://www.ncbi.nlm.nih.gov/pubmed/26469337
http://dx.doi.org/10.1371/journal.pone.0140468
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