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Ultrasensitive detection of DNA and protein markers in cancer cells
Cancer cells differ from normal cells in various parameters, and these differences are caused by genomic mutations and consequential altered gene expression. The genetic and functional heterogeneity of tumor cells is a major challenge in cancer research, detection, and effective treatment. As such,...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Chinese Anti-Cancer Association
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607821/ https://www.ncbi.nlm.nih.gov/pubmed/26487959 http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0048 |
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author | Smolina, Irina V. Broude, Natalia E. |
author_facet | Smolina, Irina V. Broude, Natalia E. |
author_sort | Smolina, Irina V. |
collection | PubMed |
description | Cancer cells differ from normal cells in various parameters, and these differences are caused by genomic mutations and consequential altered gene expression. The genetic and functional heterogeneity of tumor cells is a major challenge in cancer research, detection, and effective treatment. As such, the use of diagnostic methods is important to reveal this heterogeneity at the single-cell level. Droplet microfluidic devices are effective tools that provide exceptional sensitivity for analyzing single cells and molecules. In this review, we highlight two novel methods that employ droplet microfluidics for ultra-sensitive detection of nucleic acids and protein markers in cancer cells. We also discuss the future practical applications of these methods. |
format | Online Article Text |
id | pubmed-4607821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Chinese Anti-Cancer Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-46078212015-10-20 Ultrasensitive detection of DNA and protein markers in cancer cells Smolina, Irina V. Broude, Natalia E. Cancer Biol Med Review Cancer cells differ from normal cells in various parameters, and these differences are caused by genomic mutations and consequential altered gene expression. The genetic and functional heterogeneity of tumor cells is a major challenge in cancer research, detection, and effective treatment. As such, the use of diagnostic methods is important to reveal this heterogeneity at the single-cell level. Droplet microfluidic devices are effective tools that provide exceptional sensitivity for analyzing single cells and molecules. In this review, we highlight two novel methods that employ droplet microfluidics for ultra-sensitive detection of nucleic acids and protein markers in cancer cells. We also discuss the future practical applications of these methods. Chinese Anti-Cancer Association 2015-09 /pmc/articles/PMC4607821/ /pubmed/26487959 http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0048 Text en 2015 Cancer Biology & Medicine This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Review Smolina, Irina V. Broude, Natalia E. Ultrasensitive detection of DNA and protein markers in cancer cells |
title | Ultrasensitive detection of DNA and protein markers in cancer cells |
title_full | Ultrasensitive detection of DNA and protein markers in cancer cells |
title_fullStr | Ultrasensitive detection of DNA and protein markers in cancer cells |
title_full_unstemmed | Ultrasensitive detection of DNA and protein markers in cancer cells |
title_short | Ultrasensitive detection of DNA and protein markers in cancer cells |
title_sort | ultrasensitive detection of dna and protein markers in cancer cells |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607821/ https://www.ncbi.nlm.nih.gov/pubmed/26487959 http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0048 |
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