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Ultrasensitive detection of DNA and protein markers in cancer cells

Cancer cells differ from normal cells in various parameters, and these differences are caused by genomic mutations and consequential altered gene expression. The genetic and functional heterogeneity of tumor cells is a major challenge in cancer research, detection, and effective treatment. As such,...

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Detalles Bibliográficos
Autores principales: Smolina, Irina V., Broude, Natalia E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese Anti-Cancer Association 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607821/
https://www.ncbi.nlm.nih.gov/pubmed/26487959
http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0048
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author Smolina, Irina V.
Broude, Natalia E.
author_facet Smolina, Irina V.
Broude, Natalia E.
author_sort Smolina, Irina V.
collection PubMed
description Cancer cells differ from normal cells in various parameters, and these differences are caused by genomic mutations and consequential altered gene expression. The genetic and functional heterogeneity of tumor cells is a major challenge in cancer research, detection, and effective treatment. As such, the use of diagnostic methods is important to reveal this heterogeneity at the single-cell level. Droplet microfluidic devices are effective tools that provide exceptional sensitivity for analyzing single cells and molecules. In this review, we highlight two novel methods that employ droplet microfluidics for ultra-sensitive detection of nucleic acids and protein markers in cancer cells. We also discuss the future practical applications of these methods.
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spelling pubmed-46078212015-10-20 Ultrasensitive detection of DNA and protein markers in cancer cells Smolina, Irina V. Broude, Natalia E. Cancer Biol Med Review Cancer cells differ from normal cells in various parameters, and these differences are caused by genomic mutations and consequential altered gene expression. The genetic and functional heterogeneity of tumor cells is a major challenge in cancer research, detection, and effective treatment. As such, the use of diagnostic methods is important to reveal this heterogeneity at the single-cell level. Droplet microfluidic devices are effective tools that provide exceptional sensitivity for analyzing single cells and molecules. In this review, we highlight two novel methods that employ droplet microfluidics for ultra-sensitive detection of nucleic acids and protein markers in cancer cells. We also discuss the future practical applications of these methods. Chinese Anti-Cancer Association 2015-09 /pmc/articles/PMC4607821/ /pubmed/26487959 http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0048 Text en 2015 Cancer Biology & Medicine This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Review
Smolina, Irina V.
Broude, Natalia E.
Ultrasensitive detection of DNA and protein markers in cancer cells
title Ultrasensitive detection of DNA and protein markers in cancer cells
title_full Ultrasensitive detection of DNA and protein markers in cancer cells
title_fullStr Ultrasensitive detection of DNA and protein markers in cancer cells
title_full_unstemmed Ultrasensitive detection of DNA and protein markers in cancer cells
title_short Ultrasensitive detection of DNA and protein markers in cancer cells
title_sort ultrasensitive detection of dna and protein markers in cancer cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607821/
https://www.ncbi.nlm.nih.gov/pubmed/26487959
http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0048
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