Cargando…

Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum

OBJECTIVE: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential express...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Hua-Jun, Chen, Ya-Jing, Zuo, Duo, Xiao, Ming-Ming, Li, Ying, Guo, Hua, Zhang, Ning, Chen, Rui-Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese Anti-Cancer Association 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607824/
https://www.ncbi.nlm.nih.gov/pubmed/26487969
http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0010
_version_ 1782395560208105472
author Gao, Hua-Jun
Chen, Ya-Jing
Zuo, Duo
Xiao, Ming-Ming
Li, Ying
Guo, Hua
Zhang, Ning
Chen, Rui-Bing
author_facet Gao, Hua-Jun
Chen, Ya-Jing
Zuo, Duo
Xiao, Ming-Ming
Li, Ying
Guo, Hua
Zhang, Ning
Chen, Rui-Bing
author_sort Gao, Hua-Jun
collection PubMed
description OBJECTIVE: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples. METHODS: Lectin affinity chromatography (LAC) was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography (LC) separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the three serum proteins. RESULTS: A total of 2,280 protein groups were identified in the serum samples from HCC patients by using the 2D LC-MS/MS method. Up to 36 proteins were up-regulated in the HCC serum, whereas 19 proteins were down-regulated. Three differential glycoproteins, namely, fibrinogen gamma chain (FGG), FOS-like antigen 2 (FOSL2), and α-1,6-mannosylglycoprotein 6-β-N-acetylglucosaminyltransferase B (MGAT5B) were validated by Western blot. All these three proteins were up-regulated in the HCC serum samples. CONCLUSION: A quantitative glycoproteomic method was established and proven useful to determine potential novel biomarkers for HCC.
format Online
Article
Text
id pubmed-4607824
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Chinese Anti-Cancer Association
record_format MEDLINE/PubMed
spelling pubmed-46078242015-10-20 Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum Gao, Hua-Jun Chen, Ya-Jing Zuo, Duo Xiao, Ming-Ming Li, Ying Guo, Hua Zhang, Ning Chen, Rui-Bing Cancer Biol Med Original Article OBJECTIVE: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples. METHODS: Lectin affinity chromatography (LAC) was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography (LC) separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the three serum proteins. RESULTS: A total of 2,280 protein groups were identified in the serum samples from HCC patients by using the 2D LC-MS/MS method. Up to 36 proteins were up-regulated in the HCC serum, whereas 19 proteins were down-regulated. Three differential glycoproteins, namely, fibrinogen gamma chain (FGG), FOS-like antigen 2 (FOSL2), and α-1,6-mannosylglycoprotein 6-β-N-acetylglucosaminyltransferase B (MGAT5B) were validated by Western blot. All these three proteins were up-regulated in the HCC serum samples. CONCLUSION: A quantitative glycoproteomic method was established and proven useful to determine potential novel biomarkers for HCC. Chinese Anti-Cancer Association 2015-09 /pmc/articles/PMC4607824/ /pubmed/26487969 http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0010 Text en 2015 Cancer Biology & Medicine This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Original Article
Gao, Hua-Jun
Chen, Ya-Jing
Zuo, Duo
Xiao, Ming-Ming
Li, Ying
Guo, Hua
Zhang, Ning
Chen, Rui-Bing
Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
title Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
title_full Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
title_fullStr Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
title_full_unstemmed Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
title_short Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
title_sort quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4607824/
https://www.ncbi.nlm.nih.gov/pubmed/26487969
http://dx.doi.org/10.7497/j.issn.2095-3941.2015.0010
work_keys_str_mv AT gaohuajun quantitativeproteomicanalysisforhighthroughputscreeningofdifferentialglycoproteinsinhepatocellularcarcinomaserum
AT chenyajing quantitativeproteomicanalysisforhighthroughputscreeningofdifferentialglycoproteinsinhepatocellularcarcinomaserum
AT zuoduo quantitativeproteomicanalysisforhighthroughputscreeningofdifferentialglycoproteinsinhepatocellularcarcinomaserum
AT xiaomingming quantitativeproteomicanalysisforhighthroughputscreeningofdifferentialglycoproteinsinhepatocellularcarcinomaserum
AT liying quantitativeproteomicanalysisforhighthroughputscreeningofdifferentialglycoproteinsinhepatocellularcarcinomaserum
AT guohua quantitativeproteomicanalysisforhighthroughputscreeningofdifferentialglycoproteinsinhepatocellularcarcinomaserum
AT zhangning quantitativeproteomicanalysisforhighthroughputscreeningofdifferentialglycoproteinsinhepatocellularcarcinomaserum
AT chenruibing quantitativeproteomicanalysisforhighthroughputscreeningofdifferentialglycoproteinsinhepatocellularcarcinomaserum