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Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients

BACKGROUND: Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern. Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a...

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Autores principales: Dang, Juliet, Feng, Qinghua, Eaton, Keith D., Jang, Hona, Kiviat, Nancy B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608125/
https://www.ncbi.nlm.nih.gov/pubmed/26472641
http://dx.doi.org/10.1186/s12903-015-0111-x
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author Dang, Juliet
Feng, Qinghua
Eaton, Keith D.
Jang, Hona
Kiviat, Nancy B.
author_facet Dang, Juliet
Feng, Qinghua
Eaton, Keith D.
Jang, Hona
Kiviat, Nancy B.
author_sort Dang, Juliet
collection PubMed
description BACKGROUND: Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern. Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a clinic setting. Thus, our main objective is to study HPV detection in oral rinse samples. METHODS: In our study, we used oral rinse sample collection coupled with real-time PCR to detect for HPVs types 16 and 18, and preferentially amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients. RESULTS: Thirty three percent of 100 cancer patients were positive for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy controls was positive (this subject was positive for HPV18). CONCLUSION: Our results indicate that HPV detection in oral rinse samples may be useful as a screening tool to detect HPV-associated oral cancers.
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spelling pubmed-46081252015-10-17 Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients Dang, Juliet Feng, Qinghua Eaton, Keith D. Jang, Hona Kiviat, Nancy B. BMC Oral Health Research Article BACKGROUND: Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern. Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a clinic setting. Thus, our main objective is to study HPV detection in oral rinse samples. METHODS: In our study, we used oral rinse sample collection coupled with real-time PCR to detect for HPVs types 16 and 18, and preferentially amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients. RESULTS: Thirty three percent of 100 cancer patients were positive for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy controls was positive (this subject was positive for HPV18). CONCLUSION: Our results indicate that HPV detection in oral rinse samples may be useful as a screening tool to detect HPV-associated oral cancers. BioMed Central 2015-10-15 /pmc/articles/PMC4608125/ /pubmed/26472641 http://dx.doi.org/10.1186/s12903-015-0111-x Text en © Dang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Dang, Juliet
Feng, Qinghua
Eaton, Keith D.
Jang, Hona
Kiviat, Nancy B.
Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients
title Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients
title_full Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients
title_fullStr Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients
title_full_unstemmed Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients
title_short Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients
title_sort detection of hpv in oral rinse samples from opscc and non-opscc patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608125/
https://www.ncbi.nlm.nih.gov/pubmed/26472641
http://dx.doi.org/10.1186/s12903-015-0111-x
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