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Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates

Skin represents the largest organ of the human body and plays a crucial role in its protection from the negative impact of the outside environment, maintains its homeostasis, enables sensory interaction and thermoregulation. The traumatized skin tissue undergoes several phenotype switches due to pro...

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Autores principales: Kosikowska, Paulina, Pikula, Michal, Langa, Paulina, Trzonkowski, Piotr, Obuchowski, Michał, Lesner, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608580/
https://www.ncbi.nlm.nih.gov/pubmed/26473368
http://dx.doi.org/10.1371/journal.pone.0140377
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author Kosikowska, Paulina
Pikula, Michal
Langa, Paulina
Trzonkowski, Piotr
Obuchowski, Michał
Lesner, Adam
author_facet Kosikowska, Paulina
Pikula, Michal
Langa, Paulina
Trzonkowski, Piotr
Obuchowski, Michał
Lesner, Adam
author_sort Kosikowska, Paulina
collection PubMed
description Skin represents the largest organ of the human body and plays a crucial role in its protection from the negative impact of the outside environment, maintains its homeostasis, enables sensory interaction and thermoregulation. The traumatized skin tissue undergoes several phenotype switches due to progressive reoxygenation and release of cytokine and growth factors, that activate mechanisms of reparative processes. However, in case of wounds colonized with pathogenic microflora natural regenerative mechanisms become substantially impaired, that could lead to chronic inflammatory states with non-healing skin lesions. Herein, we present the initial results of our studies aimed at the design of bifunctional peptide-based compounds. The chemical approach, that was utilized in this work, was based on the conjugation of antimicrobial peptides with the peptides, that have potential pro-proliferative and/or cytoprotective activity towards human keratinocytes and fibroblasts, in order to obtain antimicrobials with reduced cytotoxicity or compounds that maintain both activities, i.e. inhibit bacterial or fungi growth and activate cell proliferation/migration in in vitro tests. As a result, we obtained a group of peptide conjugates that effectively inhibited the growth of selected bacterial and fungi strains and were able to stimulate proliferation and migration of keratinocytes and fibroblasts under their effective microbicidal concentrations.
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spelling pubmed-46085802015-10-29 Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates Kosikowska, Paulina Pikula, Michal Langa, Paulina Trzonkowski, Piotr Obuchowski, Michał Lesner, Adam PLoS One Research Article Skin represents the largest organ of the human body and plays a crucial role in its protection from the negative impact of the outside environment, maintains its homeostasis, enables sensory interaction and thermoregulation. The traumatized skin tissue undergoes several phenotype switches due to progressive reoxygenation and release of cytokine and growth factors, that activate mechanisms of reparative processes. However, in case of wounds colonized with pathogenic microflora natural regenerative mechanisms become substantially impaired, that could lead to chronic inflammatory states with non-healing skin lesions. Herein, we present the initial results of our studies aimed at the design of bifunctional peptide-based compounds. The chemical approach, that was utilized in this work, was based on the conjugation of antimicrobial peptides with the peptides, that have potential pro-proliferative and/or cytoprotective activity towards human keratinocytes and fibroblasts, in order to obtain antimicrobials with reduced cytotoxicity or compounds that maintain both activities, i.e. inhibit bacterial or fungi growth and activate cell proliferation/migration in in vitro tests. As a result, we obtained a group of peptide conjugates that effectively inhibited the growth of selected bacterial and fungi strains and were able to stimulate proliferation and migration of keratinocytes and fibroblasts under their effective microbicidal concentrations. Public Library of Science 2015-10-16 /pmc/articles/PMC4608580/ /pubmed/26473368 http://dx.doi.org/10.1371/journal.pone.0140377 Text en © 2015 Kosikowska et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kosikowska, Paulina
Pikula, Michal
Langa, Paulina
Trzonkowski, Piotr
Obuchowski, Michał
Lesner, Adam
Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates
title Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates
title_full Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates
title_fullStr Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates
title_full_unstemmed Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates
title_short Synthesis and Evaluation of Biological Activity of Antimicrobial – Pro-Proliferative Peptide Conjugates
title_sort synthesis and evaluation of biological activity of antimicrobial – pro-proliferative peptide conjugates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608580/
https://www.ncbi.nlm.nih.gov/pubmed/26473368
http://dx.doi.org/10.1371/journal.pone.0140377
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