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Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA

A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene th...

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Autores principales: Reddy, Teegala Lakshminarayan, Krishnarao, P Sivarama, Rao, Garikapati Koteswara, Bhimireddy, Eswar, Venkateswarlu, P, Mohapatra, Debendra K, Yadav, JS, Bhadra, Utpal, Pal Bhadra, Manika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608593/
https://www.ncbi.nlm.nih.gov/pubmed/26491299
http://dx.doi.org/10.2147/IJN.S86238
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author Reddy, Teegala Lakshminarayan
Krishnarao, P Sivarama
Rao, Garikapati Koteswara
Bhimireddy, Eswar
Venkateswarlu, P
Mohapatra, Debendra K
Yadav, JS
Bhadra, Utpal
Pal Bhadra, Manika
author_facet Reddy, Teegala Lakshminarayan
Krishnarao, P Sivarama
Rao, Garikapati Koteswara
Bhimireddy, Eswar
Venkateswarlu, P
Mohapatra, Debendra K
Yadav, JS
Bhadra, Utpal
Pal Bhadra, Manika
author_sort Reddy, Teegala Lakshminarayan
collection PubMed
description A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery.
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spelling pubmed-46085932015-10-21 Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA Reddy, Teegala Lakshminarayan Krishnarao, P Sivarama Rao, Garikapati Koteswara Bhimireddy, Eswar Venkateswarlu, P Mohapatra, Debendra K Yadav, JS Bhadra, Utpal Pal Bhadra, Manika Int J Nanomedicine Original Research A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery. Dove Medical Press 2015-10-12 /pmc/articles/PMC4608593/ /pubmed/26491299 http://dx.doi.org/10.2147/IJN.S86238 Text en © 2015 Reddy et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Reddy, Teegala Lakshminarayan
Krishnarao, P Sivarama
Rao, Garikapati Koteswara
Bhimireddy, Eswar
Venkateswarlu, P
Mohapatra, Debendra K
Yadav, JS
Bhadra, Utpal
Pal Bhadra, Manika
Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_full Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_fullStr Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_full_unstemmed Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_short Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
title_sort para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of sirna
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608593/
https://www.ncbi.nlm.nih.gov/pubmed/26491299
http://dx.doi.org/10.2147/IJN.S86238
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