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Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA
A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608593/ https://www.ncbi.nlm.nih.gov/pubmed/26491299 http://dx.doi.org/10.2147/IJN.S86238 |
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author | Reddy, Teegala Lakshminarayan Krishnarao, P Sivarama Rao, Garikapati Koteswara Bhimireddy, Eswar Venkateswarlu, P Mohapatra, Debendra K Yadav, JS Bhadra, Utpal Pal Bhadra, Manika |
author_facet | Reddy, Teegala Lakshminarayan Krishnarao, P Sivarama Rao, Garikapati Koteswara Bhimireddy, Eswar Venkateswarlu, P Mohapatra, Debendra K Yadav, JS Bhadra, Utpal Pal Bhadra, Manika |
author_sort | Reddy, Teegala Lakshminarayan |
collection | PubMed |
description | A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery. |
format | Online Article Text |
id | pubmed-4608593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46085932015-10-21 Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA Reddy, Teegala Lakshminarayan Krishnarao, P Sivarama Rao, Garikapati Koteswara Bhimireddy, Eswar Venkateswarlu, P Mohapatra, Debendra K Yadav, JS Bhadra, Utpal Pal Bhadra, Manika Int J Nanomedicine Original Research A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery. Dove Medical Press 2015-10-12 /pmc/articles/PMC4608593/ /pubmed/26491299 http://dx.doi.org/10.2147/IJN.S86238 Text en © 2015 Reddy et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Reddy, Teegala Lakshminarayan Krishnarao, P Sivarama Rao, Garikapati Koteswara Bhimireddy, Eswar Venkateswarlu, P Mohapatra, Debendra K Yadav, JS Bhadra, Utpal Pal Bhadra, Manika Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA |
title | Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA |
title_full | Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA |
title_fullStr | Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA |
title_full_unstemmed | Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA |
title_short | Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA |
title_sort | para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of sirna |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608593/ https://www.ncbi.nlm.nih.gov/pubmed/26491299 http://dx.doi.org/10.2147/IJN.S86238 |
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