The Correlation Relationship between P14(ARF) Gene DNA Methylation and Primary Liver Cancer

BACKGROUND: Primary liver cancer is a common malignant tumor that causes serious damage to human health. DNA methylation is common in epigenetics. DNA methylation plays an important role in the process of primary liver cancer occurrence and development. The P14(ARF) gene is an important tumor suppressor gene. It was found that P14(ARF) methylation is associated with the degree of malignancy in multiple tumors. Therefore, this study aimed to investigate the relationship between P14(ARF) methylation level and primary liver cancer malignant degree. MATERIAL/METHODS: Carcinoma tissues and adjacent tissues were collected from 87 primary liver cancer patients. Pyrosequencing was applied to obtain P14(ARF) methylation. Real-time PCR was used to detect P14(ARF) mRNA level. RESULTS: P14(ARF) methylation level in cancerous tissue was significantly higher than in the adjacent tissue (t=76.54, P<0.001). P14(ARF) methylation showed no significant difference in patients with different age, sex, smoking status, or drinking status. It did not present an obvious difference in tumors with different size. Its methylation level increased following the improvement of TNM stage (P<0.05). Compared with the adjacent tissue, P14(ARF) mRNA in carcinoma tissue decreased by 31% (t=28.91, P<0.001). P14(ARF) methylation showed a significant negative correlation with mRNA expression in cancerous tissue (r=−0.43, P<0.01). CONCLUSIONS: P14(ARF) mRNA level is regulated by DNA methylation in primary liver cancer. P14(ARF) gene DNA methylation may be associated with the occurrence of primary liver cancer occurrence and TNM staging.