Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium

The effect of selenium applied as sodium selenite (Na(2)SeO(3)) on the cytotoxicity of vanadyl sulphate (VOSO(4)) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na(2)SeO(3) at low doses (0.5 and 1 μM) can enhance 100 μM VOSO(4)-induced cell damage. The two-way ANOVA...

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Detalles Bibliográficos
Autor principal: Zwolak, Iwona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608973/
https://www.ncbi.nlm.nih.gov/pubmed/26201834
http://dx.doi.org/10.1007/s00128-015-1615-4
Descripción
Sumario:The effect of selenium applied as sodium selenite (Na(2)SeO(3)) on the cytotoxicity of vanadyl sulphate (VOSO(4)) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na(2)SeO(3) at low doses (0.5 and 1 μM) can enhance 100 μM VOSO(4)-induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na(2)SeO(3) with VOSO(4) and 1 μM Na(2)SeO(3) with VOSO(4). Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO(4) alone. In turn, a majority of cells co-treated with VOSO(4) and 1 μM Na(2)SeO(3) were elongated, and exhibited cytoplasmic vacuolization. These results warn of the potential contribution of inorganic selenium to vanadium-induced toxicity.

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