Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium

The effect of selenium applied as sodium selenite (Na(2)SeO(3)) on the cytotoxicity of vanadyl sulphate (VOSO(4)) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na(2)SeO(3) at low doses (0.5 and 1 μM) can enhance 100 μM VOSO(4)-induced cell damage. The two-way ANOVA...

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Autor principal: Zwolak, Iwona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608973/
https://www.ncbi.nlm.nih.gov/pubmed/26201834
http://dx.doi.org/10.1007/s00128-015-1615-4
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author Zwolak, Iwona
author_facet Zwolak, Iwona
author_sort Zwolak, Iwona
collection PubMed
description The effect of selenium applied as sodium selenite (Na(2)SeO(3)) on the cytotoxicity of vanadyl sulphate (VOSO(4)) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na(2)SeO(3) at low doses (0.5 and 1 μM) can enhance 100 μM VOSO(4)-induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na(2)SeO(3) with VOSO(4) and 1 μM Na(2)SeO(3) with VOSO(4). Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO(4) alone. In turn, a majority of cells co-treated with VOSO(4) and 1 μM Na(2)SeO(3) were elongated, and exhibited cytoplasmic vacuolization. These results warn of the potential contribution of inorganic selenium to vanadium-induced toxicity.
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spelling pubmed-46089732015-10-21 Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium Zwolak, Iwona Bull Environ Contam Toxicol Article The effect of selenium applied as sodium selenite (Na(2)SeO(3)) on the cytotoxicity of vanadyl sulphate (VOSO(4)) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na(2)SeO(3) at low doses (0.5 and 1 μM) can enhance 100 μM VOSO(4)-induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na(2)SeO(3) with VOSO(4) and 1 μM Na(2)SeO(3) with VOSO(4). Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO(4) alone. In turn, a majority of cells co-treated with VOSO(4) and 1 μM Na(2)SeO(3) were elongated, and exhibited cytoplasmic vacuolization. These results warn of the potential contribution of inorganic selenium to vanadium-induced toxicity. Springer US 2015-07-23 2015 /pmc/articles/PMC4608973/ /pubmed/26201834 http://dx.doi.org/10.1007/s00128-015-1615-4 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Zwolak, Iwona
Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium
title Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium
title_full Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium
title_fullStr Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium
title_full_unstemmed Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium
title_short Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium
title_sort increased cytotoxicity of vanadium to cho-k1 cells in the presence of inorganic selenium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608973/
https://www.ncbi.nlm.nih.gov/pubmed/26201834
http://dx.doi.org/10.1007/s00128-015-1615-4
work_keys_str_mv AT zwolakiwona increasedcytotoxicityofvanadiumtochok1cellsinthepresenceofinorganicselenium

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