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Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions
BACKGROUND: Activation of numerous protective mechanisms during cold acclimation is important for the acquisition of freezing tolerance in perennial ryegrass (Lolium perenne L.). To elucidate the molecular mechanisms of cold acclimation in two genotypes (‘Veyo’ and ‘Falster’) of perennial ryegrass f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609083/ https://www.ncbi.nlm.nih.gov/pubmed/26474965 http://dx.doi.org/10.1186/s12870-015-0643-x |
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author | Abeynayake, Shamila Weerakoon Byrne, Stephen Nagy, Istvan Jonavičienė, Kristina Etzerodt, Thomas Povl Boelt, Birte Asp, Torben |
author_facet | Abeynayake, Shamila Weerakoon Byrne, Stephen Nagy, Istvan Jonavičienė, Kristina Etzerodt, Thomas Povl Boelt, Birte Asp, Torben |
author_sort | Abeynayake, Shamila Weerakoon |
collection | PubMed |
description | BACKGROUND: Activation of numerous protective mechanisms during cold acclimation is important for the acquisition of freezing tolerance in perennial ryegrass (Lolium perenne L.). To elucidate the molecular mechanisms of cold acclimation in two genotypes (‘Veyo’ and ‘Falster’) of perennial ryegrass from distinct geographical origins, we performed transcriptome profiling during cold acclimation using RNA-Seq. METHODS: We cold-acclimated plants from both genotypes in controlled conditions for a period of 17 days and isolated Total RNA at various time points for high throughput sequencing using Illumina technology. RNA-seq reads were aligned to genotype specific references to identify transcripts with significant changes in expression during cold acclimation. RESULTS: The genes induced were involved in protective mechanisms such as cell response to abiotic stimulus, signal transduction, redox homeostasis, plasma membrane and cell wall modifications, and carbohydrate metabolism in both genotypes. ‘Falster’ genotype, adapted to cold climates, showed a stronger transcriptional differentiation during cold acclimation, and more differentially expressed transcripts related to stress, signal transduction, response to abiotic stimulus, and metabolic processes compared to ‘Veyo’. ‘Falster’ genotype also showed an induction of more transcripts with sequence homology to fructosyltransferase genes (FTs) and a higher fold induction of fructan in response to low-temperature stress. The circadian rhythm network was perturbed in the ‘Veyo’ genotype adapted to warmer climates. CONCLUSION: In this study, the differentially expressed genes during cold acclimation, potentially involved in numerous protective mechanisms, were identified in two genotypes of perennial ryegrass from distinct geographical origins. The observation that the circadian rhythm network was perturbed in ‘Veyo’ during cold acclimation may point to a low adaptability of ‘Veyo’ to low temperature stresses. This study also revealed the transcriptional mechanisms underlying carbon allocation towards fructan biosynthesis in perennial ryegrass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0643-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4609083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46090832015-10-18 Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions Abeynayake, Shamila Weerakoon Byrne, Stephen Nagy, Istvan Jonavičienė, Kristina Etzerodt, Thomas Povl Boelt, Birte Asp, Torben BMC Plant Biol Research Article BACKGROUND: Activation of numerous protective mechanisms during cold acclimation is important for the acquisition of freezing tolerance in perennial ryegrass (Lolium perenne L.). To elucidate the molecular mechanisms of cold acclimation in two genotypes (‘Veyo’ and ‘Falster’) of perennial ryegrass from distinct geographical origins, we performed transcriptome profiling during cold acclimation using RNA-Seq. METHODS: We cold-acclimated plants from both genotypes in controlled conditions for a period of 17 days and isolated Total RNA at various time points for high throughput sequencing using Illumina technology. RNA-seq reads were aligned to genotype specific references to identify transcripts with significant changes in expression during cold acclimation. RESULTS: The genes induced were involved in protective mechanisms such as cell response to abiotic stimulus, signal transduction, redox homeostasis, plasma membrane and cell wall modifications, and carbohydrate metabolism in both genotypes. ‘Falster’ genotype, adapted to cold climates, showed a stronger transcriptional differentiation during cold acclimation, and more differentially expressed transcripts related to stress, signal transduction, response to abiotic stimulus, and metabolic processes compared to ‘Veyo’. ‘Falster’ genotype also showed an induction of more transcripts with sequence homology to fructosyltransferase genes (FTs) and a higher fold induction of fructan in response to low-temperature stress. The circadian rhythm network was perturbed in the ‘Veyo’ genotype adapted to warmer climates. CONCLUSION: In this study, the differentially expressed genes during cold acclimation, potentially involved in numerous protective mechanisms, were identified in two genotypes of perennial ryegrass from distinct geographical origins. The observation that the circadian rhythm network was perturbed in ‘Veyo’ during cold acclimation may point to a low adaptability of ‘Veyo’ to low temperature stresses. This study also revealed the transcriptional mechanisms underlying carbon allocation towards fructan biosynthesis in perennial ryegrass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0643-x) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-17 /pmc/articles/PMC4609083/ /pubmed/26474965 http://dx.doi.org/10.1186/s12870-015-0643-x Text en © Abeynayake et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Abeynayake, Shamila Weerakoon Byrne, Stephen Nagy, Istvan Jonavičienė, Kristina Etzerodt, Thomas Povl Boelt, Birte Asp, Torben Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions |
title | Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions |
title_full | Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions |
title_fullStr | Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions |
title_full_unstemmed | Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions |
title_short | Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions |
title_sort | changes in lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609083/ https://www.ncbi.nlm.nih.gov/pubmed/26474965 http://dx.doi.org/10.1186/s12870-015-0643-x |
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