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Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip
BACKGROUND: Staphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. A...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609312/ https://www.ncbi.nlm.nih.gov/pubmed/26495113 http://dx.doi.org/10.5812/jjm.26793 |
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author | Gholamzad, Mehrdad Khatami, Mohammad Reza Ghassemi, Soheil Vaise Malekshahi, Ziba Shooshtari, Mohammad Barat |
author_facet | Gholamzad, Mehrdad Khatami, Mohammad Reza Ghassemi, Soheil Vaise Malekshahi, Ziba Shooshtari, Mohammad Barat |
author_sort | Gholamzad, Mehrdad |
collection | PubMed |
description | BACKGROUND: Staphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic tests because it is easy to use, requires minimal time to produce results, and does not require personnel training. OBJECTIVES: In our laboratory, the production of an immunochromatographic test strip, for the detection of SEB using a sandwich assay and a competitive method, was described; the test can detect SEB with high sensitivity. MATERIALS AND METHODS: The strip assays were compared with PCR, a valid method for detection. For PCR, a specific sequence for SEB production was detected using primers designed according to GenBank sequences. RESULTS: In total, 80 food samples suspected of SEB contamination were assessed using the two methods. Fifty-four samples were contaminated based on the PCR technique and twenty-six of those were confirmed using the strip assay. CONCLUSIONS: The sensitivity of the sandwich method was approximately 10 ng/mL and that of the competitive method was approximately 250 ng/mL. In the LFD, a highly specific monoclonal antibody used for both the sandwich and competitive methods resulted in an increased sensitivity and accuracy for the detection of a minimal SEB concentration. |
format | Online Article Text |
id | pubmed-4609312 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-46093122015-10-22 Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip Gholamzad, Mehrdad Khatami, Mohammad Reza Ghassemi, Soheil Vaise Malekshahi, Ziba Shooshtari, Mohammad Barat Jundishapur J Microbiol Research Article BACKGROUND: Staphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic tests because it is easy to use, requires minimal time to produce results, and does not require personnel training. OBJECTIVES: In our laboratory, the production of an immunochromatographic test strip, for the detection of SEB using a sandwich assay and a competitive method, was described; the test can detect SEB with high sensitivity. MATERIALS AND METHODS: The strip assays were compared with PCR, a valid method for detection. For PCR, a specific sequence for SEB production was detected using primers designed according to GenBank sequences. RESULTS: In total, 80 food samples suspected of SEB contamination were assessed using the two methods. Fifty-four samples were contaminated based on the PCR technique and twenty-six of those were confirmed using the strip assay. CONCLUSIONS: The sensitivity of the sandwich method was approximately 10 ng/mL and that of the competitive method was approximately 250 ng/mL. In the LFD, a highly specific monoclonal antibody used for both the sandwich and competitive methods resulted in an increased sensitivity and accuracy for the detection of a minimal SEB concentration. Kowsar 2015-09-08 /pmc/articles/PMC4609312/ /pubmed/26495113 http://dx.doi.org/10.5812/jjm.26793 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Research Article Gholamzad, Mehrdad Khatami, Mohammad Reza Ghassemi, Soheil Vaise Malekshahi, Ziba Shooshtari, Mohammad Barat Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip |
title | Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip |
title_full | Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip |
title_fullStr | Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip |
title_full_unstemmed | Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip |
title_short | Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip |
title_sort | detection of staphylococcus enterotoxin b (seb) using an immunochromatographic test strip |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609312/ https://www.ncbi.nlm.nih.gov/pubmed/26495113 http://dx.doi.org/10.5812/jjm.26793 |
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