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Vascular Damage in Resistant Hypertension: TNF-Alpha Inhibition Effects on Endothelial Cells
Inflammatory cytokines have been associated with the pathophysiology of hypertension and target organ damage (TOD). Resistant hypertensive patients (RHTN) are characterized by poor blood pressure control and higher prevalence of TOD. This study evaluated the relationship between plasma levels of TNF...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609371/ https://www.ncbi.nlm.nih.gov/pubmed/26504819 http://dx.doi.org/10.1155/2015/631594 |
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author | Barbaro, Natália Ruggeri de Araújo, Thiago Matos Tanus-Santos, José Eduardo Anhê, Gabriel Forato Fontana, Vanessa Moreno, Heitor |
author_facet | Barbaro, Natália Ruggeri de Araújo, Thiago Matos Tanus-Santos, José Eduardo Anhê, Gabriel Forato Fontana, Vanessa Moreno, Heitor |
author_sort | Barbaro, Natália Ruggeri |
collection | PubMed |
description | Inflammatory cytokines have been associated with the pathophysiology of hypertension and target organ damage (TOD). Resistant hypertensive patients (RHTN) are characterized by poor blood pressure control and higher prevalence of TOD. This study evaluated the relationship between plasma levels of TNF-α and arterial stiffness (pulse wave velocity—PWV) in 32 RHTN and 19 normotensive subjects. Moreover, we investigated the effect of TNF-α inhibition on human endothelial cells (HUVECs) incubated with serum from RHTN and normotensive subjects. HUVECs containing serum obtained from normotensive (n = 8) and hypertensive (n = 8) individuals were treated with TNF-α inhibitor (infliximab). Cell suspensions were used for measurement of DNA fragmentation and reactive oxygen species (ROS) content. RHTN patients showed higher levels of TNF-α compared to normotensive subjects, as well as higher PWV. Positive correlation was found between TNF-α levels and PWV measures in the whole group. HUVECs incubated with serum from RHTN showed increased cell apoptosis and higher ROS content compared to normotensive subjects. Infliximab attenuated the apoptosis of HUVECs incubated with serum from RHTN, but no effect in ROS production was observed. Our findings suggest that TNF-α might mediate, at least in part, vascular damage in resistant hypertension. |
format | Online Article Text |
id | pubmed-4609371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46093712015-10-26 Vascular Damage in Resistant Hypertension: TNF-Alpha Inhibition Effects on Endothelial Cells Barbaro, Natália Ruggeri de Araújo, Thiago Matos Tanus-Santos, José Eduardo Anhê, Gabriel Forato Fontana, Vanessa Moreno, Heitor Biomed Res Int Research Article Inflammatory cytokines have been associated with the pathophysiology of hypertension and target organ damage (TOD). Resistant hypertensive patients (RHTN) are characterized by poor blood pressure control and higher prevalence of TOD. This study evaluated the relationship between plasma levels of TNF-α and arterial stiffness (pulse wave velocity—PWV) in 32 RHTN and 19 normotensive subjects. Moreover, we investigated the effect of TNF-α inhibition on human endothelial cells (HUVECs) incubated with serum from RHTN and normotensive subjects. HUVECs containing serum obtained from normotensive (n = 8) and hypertensive (n = 8) individuals were treated with TNF-α inhibitor (infliximab). Cell suspensions were used for measurement of DNA fragmentation and reactive oxygen species (ROS) content. RHTN patients showed higher levels of TNF-α compared to normotensive subjects, as well as higher PWV. Positive correlation was found between TNF-α levels and PWV measures in the whole group. HUVECs incubated with serum from RHTN showed increased cell apoptosis and higher ROS content compared to normotensive subjects. Infliximab attenuated the apoptosis of HUVECs incubated with serum from RHTN, but no effect in ROS production was observed. Our findings suggest that TNF-α might mediate, at least in part, vascular damage in resistant hypertension. Hindawi Publishing Corporation 2015 2015-10-04 /pmc/articles/PMC4609371/ /pubmed/26504819 http://dx.doi.org/10.1155/2015/631594 Text en Copyright © 2015 Natália Ruggeri Barbaro et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Barbaro, Natália Ruggeri de Araújo, Thiago Matos Tanus-Santos, José Eduardo Anhê, Gabriel Forato Fontana, Vanessa Moreno, Heitor Vascular Damage in Resistant Hypertension: TNF-Alpha Inhibition Effects on Endothelial Cells |
title | Vascular Damage in Resistant Hypertension: TNF-Alpha Inhibition
Effects on Endothelial Cells |
title_full | Vascular Damage in Resistant Hypertension: TNF-Alpha Inhibition
Effects on Endothelial Cells |
title_fullStr | Vascular Damage in Resistant Hypertension: TNF-Alpha Inhibition
Effects on Endothelial Cells |
title_full_unstemmed | Vascular Damage in Resistant Hypertension: TNF-Alpha Inhibition
Effects on Endothelial Cells |
title_short | Vascular Damage in Resistant Hypertension: TNF-Alpha Inhibition
Effects on Endothelial Cells |
title_sort | vascular damage in resistant hypertension: tnf-alpha inhibition
effects on endothelial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609371/ https://www.ncbi.nlm.nih.gov/pubmed/26504819 http://dx.doi.org/10.1155/2015/631594 |
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