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Fluconazole Resistance Candida albicans in Females With Recurrent Vaginitis and Pir1 Overexpression
BACKGROUND: Some genes may be associated with Candida albicans resistance to azoles. Pir1 gene is described as responsible to induce resistance in C. albicans. OBJECTIVES: The current study aimed to find the relationship between fluconazole resistance and Pir1 protein (Pir1p) overexpression in the f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609386/ https://www.ncbi.nlm.nih.gov/pubmed/26495107 http://dx.doi.org/10.5812/jjm.21468 |
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author | Nasrollahi, Zahra Yadegari, Mohammad Hossein Roudbar Mohammadi, Shahla Roudbary, Maryam Hosseini Poor, Maryam Nikoomanesh, Fatemeh Rajabi Bazl, Masumeh |
author_facet | Nasrollahi, Zahra Yadegari, Mohammad Hossein Roudbar Mohammadi, Shahla Roudbary, Maryam Hosseini Poor, Maryam Nikoomanesh, Fatemeh Rajabi Bazl, Masumeh |
author_sort | Nasrollahi, Zahra |
collection | PubMed |
description | BACKGROUND: Some genes may be associated with Candida albicans resistance to azoles. Pir1 gene is described as responsible to induce resistance in C. albicans. OBJECTIVES: The current study aimed to find the relationship between fluconazole resistance and Pir1 protein (Pir1p) overexpression in the females with recurrent C. albicans vaginitis requiring longer fluconazole therapy. PATIENTS AND METHODS: A total of 52l vaginal samples were obtained from the females with C. albicans vaginitis. The azole susceptibility phenotype was determined according to the Clinical Laboratory for Standards Institute (CLSI) protocol for disk diffusion method and inhibition zone for fluconazole. Expression of pir1 gene and fluconazole -resistance were evaluated using polymerase chain reaction (PCR) in C. albicans. RESULTS: In the 52 isolates, 49 (94%) were resistant to fluconazole. Overexpression of Pir1 gene was detected in 47 (96%) fluconazole-resistant C. albicans isolates. CONCLUSIONS: The findings show fluconazole -resistance in C. albicans isolates with overexpression of Pir1p. |
format | Online Article Text |
id | pubmed-4609386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-46093862015-10-22 Fluconazole Resistance Candida albicans in Females With Recurrent Vaginitis and Pir1 Overexpression Nasrollahi, Zahra Yadegari, Mohammad Hossein Roudbar Mohammadi, Shahla Roudbary, Maryam Hosseini Poor, Maryam Nikoomanesh, Fatemeh Rajabi Bazl, Masumeh Jundishapur J Microbiol Research Article BACKGROUND: Some genes may be associated with Candida albicans resistance to azoles. Pir1 gene is described as responsible to induce resistance in C. albicans. OBJECTIVES: The current study aimed to find the relationship between fluconazole resistance and Pir1 protein (Pir1p) overexpression in the females with recurrent C. albicans vaginitis requiring longer fluconazole therapy. PATIENTS AND METHODS: A total of 52l vaginal samples were obtained from the females with C. albicans vaginitis. The azole susceptibility phenotype was determined according to the Clinical Laboratory for Standards Institute (CLSI) protocol for disk diffusion method and inhibition zone for fluconazole. Expression of pir1 gene and fluconazole -resistance were evaluated using polymerase chain reaction (PCR) in C. albicans. RESULTS: In the 52 isolates, 49 (94%) were resistant to fluconazole. Overexpression of Pir1 gene was detected in 47 (96%) fluconazole-resistant C. albicans isolates. CONCLUSIONS: The findings show fluconazole -resistance in C. albicans isolates with overexpression of Pir1p. Kowsar 2015-09-23 /pmc/articles/PMC4609386/ /pubmed/26495107 http://dx.doi.org/10.5812/jjm.21468 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Research Article Nasrollahi, Zahra Yadegari, Mohammad Hossein Roudbar Mohammadi, Shahla Roudbary, Maryam Hosseini Poor, Maryam Nikoomanesh, Fatemeh Rajabi Bazl, Masumeh Fluconazole Resistance Candida albicans in Females With Recurrent Vaginitis and Pir1 Overexpression |
title | Fluconazole Resistance Candida albicans in Females With Recurrent Vaginitis and Pir1 Overexpression |
title_full | Fluconazole Resistance Candida albicans in Females With Recurrent Vaginitis and Pir1 Overexpression |
title_fullStr | Fluconazole Resistance Candida albicans in Females With Recurrent Vaginitis and Pir1 Overexpression |
title_full_unstemmed | Fluconazole Resistance Candida albicans in Females With Recurrent Vaginitis and Pir1 Overexpression |
title_short | Fluconazole Resistance Candida albicans in Females With Recurrent Vaginitis and Pir1 Overexpression |
title_sort | fluconazole resistance candida albicans in females with recurrent vaginitis and pir1 overexpression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609386/ https://www.ncbi.nlm.nih.gov/pubmed/26495107 http://dx.doi.org/10.5812/jjm.21468 |
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