Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors

Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mes...

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Detalles Bibliográficos
Autores principales: Wu, Mei, Ge, Hongfeng, Li, Shue, Chu, Hailiang, Yang, Shili, Sun, Xiaoxing, Zhou, Zhenxia, Zhu, Xiongpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609456/
https://www.ncbi.nlm.nih.gov/pubmed/26504852
http://dx.doi.org/10.1155/2015/316351
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author Wu, Mei
Ge, Hongfeng
Li, Shue
Chu, Hailiang
Yang, Shili
Sun, Xiaoxing
Zhou, Zhenxia
Zhu, Xiongpeng
author_facet Wu, Mei
Ge, Hongfeng
Li, Shue
Chu, Hailiang
Yang, Shili
Sun, Xiaoxing
Zhou, Zhenxia
Zhu, Xiongpeng
author_sort Wu, Mei
collection PubMed
description Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mesenchymal stem cells; differentiation of mesenchymal stem cells was observed by oil red O and Alizarin red S staining; cell proliferation suppression was measured with MTS; IL-22 levels of cell-free supernatants were determined by ELISA. Mesenchymal stem cells inhibited the proliferation of activated CD4(+)T cells; moreover, mesenchymal stem cells immunosuppressed IL-22 by soluble cellular factors but not PGE2. These results suggest that mesenchymal stem cells may be a therapeutic strategy for patients with immune thrombocytopenia.
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spelling pubmed-46094562015-10-26 Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors Wu, Mei Ge, Hongfeng Li, Shue Chu, Hailiang Yang, Shili Sun, Xiaoxing Zhou, Zhenxia Zhu, Xiongpeng J Immunol Res Research Article Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mesenchymal stem cells; differentiation of mesenchymal stem cells was observed by oil red O and Alizarin red S staining; cell proliferation suppression was measured with MTS; IL-22 levels of cell-free supernatants were determined by ELISA. Mesenchymal stem cells inhibited the proliferation of activated CD4(+)T cells; moreover, mesenchymal stem cells immunosuppressed IL-22 by soluble cellular factors but not PGE2. These results suggest that mesenchymal stem cells may be a therapeutic strategy for patients with immune thrombocytopenia. Hindawi Publishing Corporation 2015 2015-10-04 /pmc/articles/PMC4609456/ /pubmed/26504852 http://dx.doi.org/10.1155/2015/316351 Text en Copyright © 2015 Mei Wu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wu, Mei
Ge, Hongfeng
Li, Shue
Chu, Hailiang
Yang, Shili
Sun, Xiaoxing
Zhou, Zhenxia
Zhu, Xiongpeng
Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors
title Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors
title_full Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors
title_fullStr Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors
title_full_unstemmed Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors
title_short Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors
title_sort mesenchymal stem cells immunosuppressed il-22 in patients with immune thrombocytopenia via soluble cellular factors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609456/
https://www.ncbi.nlm.nih.gov/pubmed/26504852
http://dx.doi.org/10.1155/2015/316351
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