Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry

Simultaneous identification of donkey-hide gelatin and bovine-hide gelatin in deer-horn glue was established by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry. Water containing 1% NH(4)HCO(3) was used for sample dissolution and trypsin was used for hydrolysis of the gelat...

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Autores principales: Chen, Jia, Cheng, Xian-Long, Wei, Feng, Zhang, Qian-Qian, Li, Ming-Hua, Ma, Shuang-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609468/
https://www.ncbi.nlm.nih.gov/pubmed/26504613
http://dx.doi.org/10.1155/2015/259757
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author Chen, Jia
Cheng, Xian-Long
Wei, Feng
Zhang, Qian-Qian
Li, Ming-Hua
Ma, Shuang-Cheng
author_facet Chen, Jia
Cheng, Xian-Long
Wei, Feng
Zhang, Qian-Qian
Li, Ming-Hua
Ma, Shuang-Cheng
author_sort Chen, Jia
collection PubMed
description Simultaneous identification of donkey-hide gelatin and bovine-hide gelatin in deer-horn glue was established by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry. Water containing 1% NH(4)HCO(3) was used for sample dissolution and trypsin was used for hydrolysis of the gelatins. After separation by a SB-C18 reversed-phase analytical column, collagen marker peptides were detected by mass spectrometry in positive electrospray ionization mode with multiple reaction monitoring. The method was specific, precise and reliable, and suitable for detection of adulterants derived from donkey-hide gelatin and bovine-hide gelatin in deer-horn glue.
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spelling pubmed-46094682015-10-26 Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry Chen, Jia Cheng, Xian-Long Wei, Feng Zhang, Qian-Qian Li, Ming-Hua Ma, Shuang-Cheng J Anal Methods Chem Research Article Simultaneous identification of donkey-hide gelatin and bovine-hide gelatin in deer-horn glue was established by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry. Water containing 1% NH(4)HCO(3) was used for sample dissolution and trypsin was used for hydrolysis of the gelatins. After separation by a SB-C18 reversed-phase analytical column, collagen marker peptides were detected by mass spectrometry in positive electrospray ionization mode with multiple reaction monitoring. The method was specific, precise and reliable, and suitable for detection of adulterants derived from donkey-hide gelatin and bovine-hide gelatin in deer-horn glue. Hindawi Publishing Corporation 2015 2015-10-04 /pmc/articles/PMC4609468/ /pubmed/26504613 http://dx.doi.org/10.1155/2015/259757 Text en Copyright © 2015 Jia Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Jia
Cheng, Xian-Long
Wei, Feng
Zhang, Qian-Qian
Li, Ming-Hua
Ma, Shuang-Cheng
Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_full Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_fullStr Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_full_unstemmed Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_short Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_sort detection of gelatin adulteration in traditional chinese medicine: analysis of deer-horn glue by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609468/
https://www.ncbi.nlm.nih.gov/pubmed/26504613
http://dx.doi.org/10.1155/2015/259757
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