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Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts

INTRODUCTION: The aim of this study was to evaluate the cytotoxicity of a new nano zinc-oxide eugenol (NZOE) sealer in comparison with AH-26 and Pulpdent root canal sealers. METHODS AND MATERIALS: The L929 mouse fibroblast cells were cultivated and incubated for 24, 48 or 72 h with different dilutio...

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Detalles Bibliográficos
Autores principales: Javidi, Maryam, Zarei, Mina, Omidi, Salma, Ghorbani, Ahmad, Gharechahi, Maryam, Shayani Rad, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Center for Endodontic Research 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609659/
https://www.ncbi.nlm.nih.gov/pubmed/26525834
http://dx.doi.org/10.7508/iej.2015.04.004
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author Javidi, Maryam
Zarei, Mina
Omidi, Salma
Ghorbani, Ahmad
Gharechahi, Maryam
Shayani Rad, Maryam
author_facet Javidi, Maryam
Zarei, Mina
Omidi, Salma
Ghorbani, Ahmad
Gharechahi, Maryam
Shayani Rad, Maryam
author_sort Javidi, Maryam
collection PubMed
description INTRODUCTION: The aim of this study was to evaluate the cytotoxicity of a new nano zinc-oxide eugenol (NZOE) sealer in comparison with AH-26 and Pulpdent root canal sealers. METHODS AND MATERIALS: The L929 mouse fibroblast cells were cultivated and incubated for 24, 48 or 72 h with different dilutions (1/1, 1/2, 1/4, 1/8, 1/16 and 1/32) of culture media previously exposed to either of the test sealers naming NZOE, AH-26 or Pulpdent. At the end of incubation period, the effect of sealers on cell viability was evaluated using Mosmann’s Tetrazolium Toxicity (MTT) colorimetric assay. The data was compared using the one-way analysis of variance (ANOVA) followed by the Tukey’s post hoc test for multiple comparisons. RESULTS: After 24, 48 or 72 h, both NZOE and Pulpdent sealers inhibited cell viability at 1/1, 1/2 and 1/8 dilutions. Within the 24 and 48 h, the AH-26 sealer reduced the cell viability at all dilutions except the 1/32 solution; however after 72 h even the 1/32 dilution was cytotoxic. CONCLUSION: The biocompatibility of the nano zinc-oxide eugenol sealer was comparable to Pulpdent sealer and lower than AH-26.
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spelling pubmed-46096592015-10-30 Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts Javidi, Maryam Zarei, Mina Omidi, Salma Ghorbani, Ahmad Gharechahi, Maryam Shayani Rad, Maryam Iran Endod J Original Article INTRODUCTION: The aim of this study was to evaluate the cytotoxicity of a new nano zinc-oxide eugenol (NZOE) sealer in comparison with AH-26 and Pulpdent root canal sealers. METHODS AND MATERIALS: The L929 mouse fibroblast cells were cultivated and incubated for 24, 48 or 72 h with different dilutions (1/1, 1/2, 1/4, 1/8, 1/16 and 1/32) of culture media previously exposed to either of the test sealers naming NZOE, AH-26 or Pulpdent. At the end of incubation period, the effect of sealers on cell viability was evaluated using Mosmann’s Tetrazolium Toxicity (MTT) colorimetric assay. The data was compared using the one-way analysis of variance (ANOVA) followed by the Tukey’s post hoc test for multiple comparisons. RESULTS: After 24, 48 or 72 h, both NZOE and Pulpdent sealers inhibited cell viability at 1/1, 1/2 and 1/8 dilutions. Within the 24 and 48 h, the AH-26 sealer reduced the cell viability at all dilutions except the 1/32 solution; however after 72 h even the 1/32 dilution was cytotoxic. CONCLUSION: The biocompatibility of the nano zinc-oxide eugenol sealer was comparable to Pulpdent sealer and lower than AH-26. Iranian Center for Endodontic Research 2015 /pmc/articles/PMC4609659/ /pubmed/26525834 http://dx.doi.org/10.7508/iej.2015.04.004 Text en © 2015, Iranian Center for Endodontic Research This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Javidi, Maryam
Zarei, Mina
Omidi, Salma
Ghorbani, Ahmad
Gharechahi, Maryam
Shayani Rad, Maryam
Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts
title Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts
title_full Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts
title_fullStr Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts
title_full_unstemmed Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts
title_short Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts
title_sort cytotoxicity of a new nano zinc-oxide eugenol sealer on murine fibroblasts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609659/
https://www.ncbi.nlm.nih.gov/pubmed/26525834
http://dx.doi.org/10.7508/iej.2015.04.004
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