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Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract
Purpose. To examine the promoter methylation and histone modification of WRN (Werner syndrome gene), a DNA repair gene, and their relationship with the gene expression in age-related cataract (ARC) lens. Methods. We collected the lenses after cataract surgery from 117ARC patients and 39 age-matched...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609838/ https://www.ncbi.nlm.nih.gov/pubmed/26509079 http://dx.doi.org/10.1155/2015/579695 |
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author | Zhu, Xi Zhang, Guowei Kang, Lihua Guan, Huaijin |
author_facet | Zhu, Xi Zhang, Guowei Kang, Lihua Guan, Huaijin |
author_sort | Zhu, Xi |
collection | PubMed |
description | Purpose. To examine the promoter methylation and histone modification of WRN (Werner syndrome gene), a DNA repair gene, and their relationship with the gene expression in age-related cataract (ARC) lens. Methods. We collected the lenses after cataract surgery from 117ARC patients and 39 age-matched non-ARC. WRN expression, DNA methylation and histone modification around the CpG island were assessed. The methylation status of Human-lens-epithelium cell (HLEB-3) was chemically altered to observe the relationship between methylation and expression of WRN. Results. The WRN expression was significantly decreased in the ARC anterior lens capsules comparing with the control. The CpG island of WRN promoter in the ARC anterior lens capsules displayed hypermethylation comparing with the controls. The WRN promoter was almost fully methylated in the cortex of ARC and control lens. Acetylated H3 was lower while methylated H3-K9 was higher in ARC anterior lens capsules than that of the controls. The expression of WRN in HLEB-3 increased after demethylation of the cells. Conclusions. A hypermethylation in WRN promoter and altered histone modification in anterior lens capsules might contribute to the ARC mechanism. The data suggest an association of altered DNA repair capability in lens with ARC pathogenesis. |
format | Online Article Text |
id | pubmed-4609838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46098382015-10-27 Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract Zhu, Xi Zhang, Guowei Kang, Lihua Guan, Huaijin J Ophthalmol Research Article Purpose. To examine the promoter methylation and histone modification of WRN (Werner syndrome gene), a DNA repair gene, and their relationship with the gene expression in age-related cataract (ARC) lens. Methods. We collected the lenses after cataract surgery from 117ARC patients and 39 age-matched non-ARC. WRN expression, DNA methylation and histone modification around the CpG island were assessed. The methylation status of Human-lens-epithelium cell (HLEB-3) was chemically altered to observe the relationship between methylation and expression of WRN. Results. The WRN expression was significantly decreased in the ARC anterior lens capsules comparing with the control. The CpG island of WRN promoter in the ARC anterior lens capsules displayed hypermethylation comparing with the controls. The WRN promoter was almost fully methylated in the cortex of ARC and control lens. Acetylated H3 was lower while methylated H3-K9 was higher in ARC anterior lens capsules than that of the controls. The expression of WRN in HLEB-3 increased after demethylation of the cells. Conclusions. A hypermethylation in WRN promoter and altered histone modification in anterior lens capsules might contribute to the ARC mechanism. The data suggest an association of altered DNA repair capability in lens with ARC pathogenesis. Hindawi Publishing Corporation 2015 2015-10-05 /pmc/articles/PMC4609838/ /pubmed/26509079 http://dx.doi.org/10.1155/2015/579695 Text en Copyright © 2015 Xi Zhu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhu, Xi Zhang, Guowei Kang, Lihua Guan, Huaijin Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract |
title | Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract |
title_full | Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract |
title_fullStr | Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract |
title_full_unstemmed | Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract |
title_short | Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract |
title_sort | epigenetic regulation of werner syndrome gene in age-related cataract |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609838/ https://www.ncbi.nlm.nih.gov/pubmed/26509079 http://dx.doi.org/10.1155/2015/579695 |
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