Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells

Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSE...

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Autores principales: Ojima, Takuma, Shibata, Eri, Saito, Shiho, Toyoda, Masashi, Nakajima, Hideki, Yamazaki-Inoue, Mayu, Miyagawa, Yoshitaka, Kiyokawa, Nobutaka, Fujimoto, Jun-ichiro, Sato, Toshinori, Umezawa, Akihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609952/
https://www.ncbi.nlm.nih.gov/pubmed/26477663
http://dx.doi.org/10.1038/srep14988
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author Ojima, Takuma
Shibata, Eri
Saito, Shiho
Toyoda, Masashi
Nakajima, Hideki
Yamazaki-Inoue, Mayu
Miyagawa, Yoshitaka
Kiyokawa, Nobutaka
Fujimoto, Jun-ichiro
Sato, Toshinori
Umezawa, Akihiro
author_facet Ojima, Takuma
Shibata, Eri
Saito, Shiho
Toyoda, Masashi
Nakajima, Hideki
Yamazaki-Inoue, Mayu
Miyagawa, Yoshitaka
Kiyokawa, Nobutaka
Fujimoto, Jun-ichiro
Sato, Toshinori
Umezawa, Akihiro
author_sort Ojima, Takuma
collection PubMed
description Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells’ genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells.
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spelling pubmed-46099522015-10-29 Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells Ojima, Takuma Shibata, Eri Saito, Shiho Toyoda, Masashi Nakajima, Hideki Yamazaki-Inoue, Mayu Miyagawa, Yoshitaka Kiyokawa, Nobutaka Fujimoto, Jun-ichiro Sato, Toshinori Umezawa, Akihiro Sci Rep Article Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells’ genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells. Nature Publishing Group 2015-10-19 /pmc/articles/PMC4609952/ /pubmed/26477663 http://dx.doi.org/10.1038/srep14988 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ojima, Takuma
Shibata, Eri
Saito, Shiho
Toyoda, Masashi
Nakajima, Hideki
Yamazaki-Inoue, Mayu
Miyagawa, Yoshitaka
Kiyokawa, Nobutaka
Fujimoto, Jun-ichiro
Sato, Toshinori
Umezawa, Akihiro
Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
title Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
title_full Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
title_fullStr Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
title_full_unstemmed Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
title_short Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
title_sort glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609952/
https://www.ncbi.nlm.nih.gov/pubmed/26477663
http://dx.doi.org/10.1038/srep14988
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