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Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae

Rearrangement of genomic DNA elements in a dynamic controlled fashion is a fundamental challenge. Site-specific DNA recombinases have been tamed as a powerful tool in genome editing. Here, we reported a DNA element rearrangement on the basis of a pairwise orthogonal recombination system which is com...

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Autores principales: Lin, Qiuhui, Qi, Hao, Wu, Yi, Yuan, Yingjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609996/
https://www.ncbi.nlm.nih.gov/pubmed/26477943
http://dx.doi.org/10.1038/srep15249
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author Lin, Qiuhui
Qi, Hao
Wu, Yi
Yuan, Yingjin
author_facet Lin, Qiuhui
Qi, Hao
Wu, Yi
Yuan, Yingjin
author_sort Lin, Qiuhui
collection PubMed
description Rearrangement of genomic DNA elements in a dynamic controlled fashion is a fundamental challenge. Site-specific DNA recombinases have been tamed as a powerful tool in genome editing. Here, we reported a DNA element rearrangement on the basis of a pairwise orthogonal recombination system which is comprised of two site-specific recombinases of Vika/vox and Cre/loxp in yeast Saccharomyces Creevisiae. Taking the advantage of the robust pairwise orthogonality, we showed that multi gene elements could be organized in a programmed way, in which rationally designed pattern of loxP and vox determined the final genotype after expressing corresponding recombinases. Finally, it was demonstrated that the pairwise orthogonal recombination system could be utilized to refine synthetic chromosome rearrangement and modification by loxP-mediated evolution, SCRaMbLE, in yeast cell carrying a completely synthesized chromosome III.
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spelling pubmed-46099962015-10-29 Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae Lin, Qiuhui Qi, Hao Wu, Yi Yuan, Yingjin Sci Rep Article Rearrangement of genomic DNA elements in a dynamic controlled fashion is a fundamental challenge. Site-specific DNA recombinases have been tamed as a powerful tool in genome editing. Here, we reported a DNA element rearrangement on the basis of a pairwise orthogonal recombination system which is comprised of two site-specific recombinases of Vika/vox and Cre/loxp in yeast Saccharomyces Creevisiae. Taking the advantage of the robust pairwise orthogonality, we showed that multi gene elements could be organized in a programmed way, in which rationally designed pattern of loxP and vox determined the final genotype after expressing corresponding recombinases. Finally, it was demonstrated that the pairwise orthogonal recombination system could be utilized to refine synthetic chromosome rearrangement and modification by loxP-mediated evolution, SCRaMbLE, in yeast cell carrying a completely synthesized chromosome III. Nature Publishing Group 2015-10-19 /pmc/articles/PMC4609996/ /pubmed/26477943 http://dx.doi.org/10.1038/srep15249 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lin, Qiuhui
Qi, Hao
Wu, Yi
Yuan, Yingjin
Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae
title Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae
title_full Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae
title_fullStr Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae
title_full_unstemmed Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae
title_short Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces Cerevisiae
title_sort robust orthogonal recombination system for versatile genomic elements rearrangement in yeast saccharomyces cerevisiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609996/
https://www.ncbi.nlm.nih.gov/pubmed/26477943
http://dx.doi.org/10.1038/srep15249
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