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Interaction of Isoflavones and Endophyte-Infected Tall Fescue Seed Extract on Vasoactivity of Bovine Mesenteric Vasculature
It was hypothesized that isoflavones may attenuate ergot alkaloid-induced vasoconstriction and possibly alleviate diminished contractility of vasculature after exposure to ergot alkaloids. The objective of this study was to determine if prior incubation of bovine mesenteric vasculature with the isof...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610206/ https://www.ncbi.nlm.nih.gov/pubmed/26539437 http://dx.doi.org/10.3389/fnut.2015.00032 |
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author | Jia, Yang Harmon, David L. Flythe, Michael D. Klotz, James L. |
author_facet | Jia, Yang Harmon, David L. Flythe, Michael D. Klotz, James L. |
author_sort | Jia, Yang |
collection | PubMed |
description | It was hypothesized that isoflavones may attenuate ergot alkaloid-induced vasoconstriction and possibly alleviate diminished contractility of vasculature after exposure to ergot alkaloids. The objective of this study was to determine if prior incubation of bovine mesenteric vasculature with the isoflavones formononetin (F), biochanin A (B), or an ergovaline-containing tall fescue seed extract (EXT) and their combinations affect ergotamine (ERT)-induced contractility. Multiple segments of mesenteric artery and vein supporting the ileal flange of the small intestine were collected from Angus heifers at slaughter (n = 5, bodyweight = 639 ± 39 kg). Duplicates of each vessel type were incubated in tissue culture flasks at 37°C with a 50-mL volume of Krebs–Henseleit buffer containing: only buffer (control); or 1 × 10(−6) M EXT; F; or B; and combinations of 1 × 10(−6) M EXT + F; 1 × 10(−6) M EXT + B; 1 × 10(−6) M F + B; or 1 × 10(−6) M EXT + F + B. After incubation for 2 h, sections were mounted in a multimyograph chamber. The ERT dose responses were normalized to 0.12 M KCl. Pretreatment with F, B, and F + B without EXT resulted in similar contractile responses to ERT in mesenteric artery and all incubations containing EXT resulted in a complete loss of vasoactivity to ERT. In mesenteric artery pretreated with EXT, treatments that contained B had higher contractile responses (P < 0.05) at ERT concentrations of 1 × 10(−7) and 5 × 10(−7) M. Also, treatments containing B tended (P < 0.1) to have greater responses than treatments without B at ERT concentrations of 1 × 10(−6), 5 × 10(−6), and 5 × 10(−5) M. In mesenteric vein pretreated with EXT, treatments containing F had greater contractile responses to ERT at 1 × 10(−5), 5 × 10(−5), and 1 × 10(−4) M (P < 0.05). These data indicated that F and B at 1 × 10(−6) M and their combination did not impact the overall contractile response to ERT in mesenteric vasculature. However, F and B may offset some of the vasoconstriction caused by prior exposure to ergot alkaloids. |
format | Online Article Text |
id | pubmed-4610206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46102062015-11-04 Interaction of Isoflavones and Endophyte-Infected Tall Fescue Seed Extract on Vasoactivity of Bovine Mesenteric Vasculature Jia, Yang Harmon, David L. Flythe, Michael D. Klotz, James L. Front Nutr Nutrition It was hypothesized that isoflavones may attenuate ergot alkaloid-induced vasoconstriction and possibly alleviate diminished contractility of vasculature after exposure to ergot alkaloids. The objective of this study was to determine if prior incubation of bovine mesenteric vasculature with the isoflavones formononetin (F), biochanin A (B), or an ergovaline-containing tall fescue seed extract (EXT) and their combinations affect ergotamine (ERT)-induced contractility. Multiple segments of mesenteric artery and vein supporting the ileal flange of the small intestine were collected from Angus heifers at slaughter (n = 5, bodyweight = 639 ± 39 kg). Duplicates of each vessel type were incubated in tissue culture flasks at 37°C with a 50-mL volume of Krebs–Henseleit buffer containing: only buffer (control); or 1 × 10(−6) M EXT; F; or B; and combinations of 1 × 10(−6) M EXT + F; 1 × 10(−6) M EXT + B; 1 × 10(−6) M F + B; or 1 × 10(−6) M EXT + F + B. After incubation for 2 h, sections were mounted in a multimyograph chamber. The ERT dose responses were normalized to 0.12 M KCl. Pretreatment with F, B, and F + B without EXT resulted in similar contractile responses to ERT in mesenteric artery and all incubations containing EXT resulted in a complete loss of vasoactivity to ERT. In mesenteric artery pretreated with EXT, treatments that contained B had higher contractile responses (P < 0.05) at ERT concentrations of 1 × 10(−7) and 5 × 10(−7) M. Also, treatments containing B tended (P < 0.1) to have greater responses than treatments without B at ERT concentrations of 1 × 10(−6), 5 × 10(−6), and 5 × 10(−5) M. In mesenteric vein pretreated with EXT, treatments containing F had greater contractile responses to ERT at 1 × 10(−5), 5 × 10(−5), and 1 × 10(−4) M (P < 0.05). These data indicated that F and B at 1 × 10(−6) M and their combination did not impact the overall contractile response to ERT in mesenteric vasculature. However, F and B may offset some of the vasoconstriction caused by prior exposure to ergot alkaloids. Frontiers Media S.A. 2015-10-19 /pmc/articles/PMC4610206/ /pubmed/26539437 http://dx.doi.org/10.3389/fnut.2015.00032 Text en Copyright © 2015 Jia, Harmon, Flythe and Klotz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Jia, Yang Harmon, David L. Flythe, Michael D. Klotz, James L. Interaction of Isoflavones and Endophyte-Infected Tall Fescue Seed Extract on Vasoactivity of Bovine Mesenteric Vasculature |
title | Interaction of Isoflavones and Endophyte-Infected Tall Fescue Seed Extract on Vasoactivity of Bovine Mesenteric Vasculature |
title_full | Interaction of Isoflavones and Endophyte-Infected Tall Fescue Seed Extract on Vasoactivity of Bovine Mesenteric Vasculature |
title_fullStr | Interaction of Isoflavones and Endophyte-Infected Tall Fescue Seed Extract on Vasoactivity of Bovine Mesenteric Vasculature |
title_full_unstemmed | Interaction of Isoflavones and Endophyte-Infected Tall Fescue Seed Extract on Vasoactivity of Bovine Mesenteric Vasculature |
title_short | Interaction of Isoflavones and Endophyte-Infected Tall Fescue Seed Extract on Vasoactivity of Bovine Mesenteric Vasculature |
title_sort | interaction of isoflavones and endophyte-infected tall fescue seed extract on vasoactivity of bovine mesenteric vasculature |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610206/ https://www.ncbi.nlm.nih.gov/pubmed/26539437 http://dx.doi.org/10.3389/fnut.2015.00032 |
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