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Characterization and transferability of microsatellite markers developed for Carpinus betulus (Betulaceae)(1)

PREMISE OF THE STUDY: Carpinus betulus (Betulaceae) is an octoploid, ecologically important, common tree species in European woodlands. We established 11 nuclear microsatellite loci allowing for detailed analyses of genetic diversity and structure. METHODS AND RESULTS: A microsatellite-enriched libr...

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Detalles Bibliográficos
Autores principales: Prinz, Kathleen, Finkeldey, Reiner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610309/
https://www.ncbi.nlm.nih.gov/pubmed/26504678
http://dx.doi.org/10.3732/apps.1500053
Descripción
Sumario:PREMISE OF THE STUDY: Carpinus betulus (Betulaceae) is an octoploid, ecologically important, common tree species in European woodlands. We established 11 nuclear microsatellite loci allowing for detailed analyses of genetic diversity and structure. METHODS AND RESULTS: A microsatellite-enriched library was used to develop primers for 11 microsatellite loci that revealed high allele numbers and genetic diversity in a preliminary study. CONCLUSIONS: All of the loci developed here are informative for C. betulus. In addition, the loci are transferable to several species within the genus, and almost all loci cross-amplified in species of different genera of the Betulaceae.