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Isolation and characterization of microsatellite loci in Rehmannia glutinosa (Scrophulariaceae), a medicinal herb(1)

PREMISE OF THE STUDY: Rehmannia glutinosa (Scrophulariaceae) is used in traditional Chinese medicine. Microsatellite primers were developed and characterized for this species to evaluate its genetic diversity and population genetic structure. METHODS AND RESULTS: Sixteen microsatellite loci were iso...

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Autores principales: Jiao, Zhenbin, Cheng, Yueqin, Wang, Hongwei, Lei, Caiyan, Wang, G. Geoff, Han, Linjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610310/
https://www.ncbi.nlm.nih.gov/pubmed/26504679
http://dx.doi.org/10.3732/apps.1500054
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author Jiao, Zhenbin
Cheng, Yueqin
Wang, Hongwei
Lei, Caiyan
Wang, G. Geoff
Han, Linjiang
author_facet Jiao, Zhenbin
Cheng, Yueqin
Wang, Hongwei
Lei, Caiyan
Wang, G. Geoff
Han, Linjiang
author_sort Jiao, Zhenbin
collection PubMed
description PREMISE OF THE STUDY: Rehmannia glutinosa (Scrophulariaceae) is used in traditional Chinese medicine. Microsatellite primers were developed and characterized for this species to evaluate its genetic diversity and population genetic structure. METHODS AND RESULTS: Sixteen microsatellite loci were isolated from R. glutinosa using an enriched genomic library, and these markers were characterized in two wild populations of this species. The number of alleles per locus ranged from two to 20. A high genetic diversity was observed in two populations, with average observed heterozygosity of 0.812 and 0.794, and average expected heterozygosity of 0.802 and 0.814, respectively. CONCLUSIONS: Rehmannia glutinosa is an important medicinal resource. The genetic markers described in our study will be useful for future population genetic studies and molecular breeding programs on this species.
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spelling pubmed-46103102015-10-26 Isolation and characterization of microsatellite loci in Rehmannia glutinosa (Scrophulariaceae), a medicinal herb(1) Jiao, Zhenbin Cheng, Yueqin Wang, Hongwei Lei, Caiyan Wang, G. Geoff Han, Linjiang Appl Plant Sci Primer Note PREMISE OF THE STUDY: Rehmannia glutinosa (Scrophulariaceae) is used in traditional Chinese medicine. Microsatellite primers were developed and characterized for this species to evaluate its genetic diversity and population genetic structure. METHODS AND RESULTS: Sixteen microsatellite loci were isolated from R. glutinosa using an enriched genomic library, and these markers were characterized in two wild populations of this species. The number of alleles per locus ranged from two to 20. A high genetic diversity was observed in two populations, with average observed heterozygosity of 0.812 and 0.794, and average expected heterozygosity of 0.802 and 0.814, respectively. CONCLUSIONS: Rehmannia glutinosa is an important medicinal resource. The genetic markers described in our study will be useful for future population genetic studies and molecular breeding programs on this species. Botanical Society of America 2015-10-13 /pmc/articles/PMC4610310/ /pubmed/26504679 http://dx.doi.org/10.3732/apps.1500054 Text en © 2015 Jiao et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Jiao, Zhenbin
Cheng, Yueqin
Wang, Hongwei
Lei, Caiyan
Wang, G. Geoff
Han, Linjiang
Isolation and characterization of microsatellite loci in Rehmannia glutinosa (Scrophulariaceae), a medicinal herb(1)
title Isolation and characterization of microsatellite loci in Rehmannia glutinosa (Scrophulariaceae), a medicinal herb(1)
title_full Isolation and characterization of microsatellite loci in Rehmannia glutinosa (Scrophulariaceae), a medicinal herb(1)
title_fullStr Isolation and characterization of microsatellite loci in Rehmannia glutinosa (Scrophulariaceae), a medicinal herb(1)
title_full_unstemmed Isolation and characterization of microsatellite loci in Rehmannia glutinosa (Scrophulariaceae), a medicinal herb(1)
title_short Isolation and characterization of microsatellite loci in Rehmannia glutinosa (Scrophulariaceae), a medicinal herb(1)
title_sort isolation and characterization of microsatellite loci in rehmannia glutinosa (scrophulariaceae), a medicinal herb(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610310/
https://www.ncbi.nlm.nih.gov/pubmed/26504679
http://dx.doi.org/10.3732/apps.1500054
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