Isolation of nuclear microsatellites in the African timber tree Lophira alata (Ochnaceae) and cross-amplification in L. lanceolata(1)

PREMISE OF THE STUDY: Microsatellite markers were isolated in the rainforest tree Lophira alata (Ochnaceae), an important timber tree from Central Africa, and cross-amplified on its savannah counterpart, L. lanceolata. METHODS AND RESULTS: From a microsatellite-enriched library sequenced on a 454 GS...

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Detalles Bibliográficos
Autores principales: Piñeiro, Rosalía, Staquet, Adrien, Hardy, Olivier J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610311/
https://www.ncbi.nlm.nih.gov/pubmed/26504680
http://dx.doi.org/10.3732/apps.1500056
Descripción
Sumario:PREMISE OF THE STUDY: Microsatellite markers were isolated in the rainforest tree Lophira alata (Ochnaceae), an important timber tree from Central Africa, and cross-amplified on its savannah counterpart, L. lanceolata. METHODS AND RESULTS: From a microsatellite-enriched library sequenced on a 454 GS FLX platform, 13 primer combinations were identified. Amplification was optimized in two multiplex reactions. The primers amplified di- and trinucelotide repeats, with two to seven alleles per locus. Eleven primers also amplified in L. lanceolata. CONCLUSIONS: Microsatellite primers developed for the genus Lophira displayed sufficient variation to investigate hybridization between congeneric species in the rainforest–savannah transition.

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