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“Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity

In our efforts toward producing environmentally responsible but highly stable bioelectrodes with high electroactivities, we report here a simple, inexpensive, autoclavable high sensitivity biosensor based on enzyme-polymer nanogels. Met-hemoglobin (Hb) is stabilized by wrapping it in high molecular...

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Autores principales: Ghimire, Ananta, Zore, Omkar V., Thilakarathne, Vindya K., Briand, Victoria A., Lenehan, Patrick J., Lei, Yu, Kasi, Rajeswari M., Kumar, Challa V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610568/
https://www.ncbi.nlm.nih.gov/pubmed/26393601
http://dx.doi.org/10.3390/s150923868
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author Ghimire, Ananta
Zore, Omkar V.
Thilakarathne, Vindya K.
Briand, Victoria A.
Lenehan, Patrick J.
Lei, Yu
Kasi, Rajeswari M.
Kumar, Challa V.
author_facet Ghimire, Ananta
Zore, Omkar V.
Thilakarathne, Vindya K.
Briand, Victoria A.
Lenehan, Patrick J.
Lei, Yu
Kasi, Rajeswari M.
Kumar, Challa V.
author_sort Ghimire, Ananta
collection PubMed
description In our efforts toward producing environmentally responsible but highly stable bioelectrodes with high electroactivities, we report here a simple, inexpensive, autoclavable high sensitivity biosensor based on enzyme-polymer nanogels. Met-hemoglobin (Hb) is stabilized by wrapping it in high molecular weight poly(acrylic acid) (PAA, M(W) 450k), and the resulting nanogels abbreviated as Hb-PAA-450k, withstood exposure to high temperatures for extended periods under steam sterilization conditions (122 °C, 10 min, 17–20 psi) without loss of Hb structure or its peroxidase-like activities. The bioelectrodes prepared by coating Hb-PAA-450k nanogels on glassy carbon showed well-defined quasi-reversible redox peaks at −0.279 and −0.334 V in cyclic voltammetry (CV) and retained >95% electroactivity after storing for 14 days at room temperature. Similarly, the bioelectrode showed ~90% retention in electrochemical properties after autoclaving under steam sterilization conditions. The ultra stable bioelectrode was used to detect hydrogen peroxide and demonstrated an excellent detection limit of 0.5 μM, the best among the Hb-based electrochemical biosensors. This is the first electrochemical demonstration of steam-sterilizable, storable, modular bioelectrode that undergoes reversible-thermal denaturation and retains electroactivity for protein based electrochemical applications.
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spelling pubmed-46105682015-10-26 “Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity Ghimire, Ananta Zore, Omkar V. Thilakarathne, Vindya K. Briand, Victoria A. Lenehan, Patrick J. Lei, Yu Kasi, Rajeswari M. Kumar, Challa V. Sensors (Basel) Article In our efforts toward producing environmentally responsible but highly stable bioelectrodes with high electroactivities, we report here a simple, inexpensive, autoclavable high sensitivity biosensor based on enzyme-polymer nanogels. Met-hemoglobin (Hb) is stabilized by wrapping it in high molecular weight poly(acrylic acid) (PAA, M(W) 450k), and the resulting nanogels abbreviated as Hb-PAA-450k, withstood exposure to high temperatures for extended periods under steam sterilization conditions (122 °C, 10 min, 17–20 psi) without loss of Hb structure or its peroxidase-like activities. The bioelectrodes prepared by coating Hb-PAA-450k nanogels on glassy carbon showed well-defined quasi-reversible redox peaks at −0.279 and −0.334 V in cyclic voltammetry (CV) and retained >95% electroactivity after storing for 14 days at room temperature. Similarly, the bioelectrode showed ~90% retention in electrochemical properties after autoclaving under steam sterilization conditions. The ultra stable bioelectrode was used to detect hydrogen peroxide and demonstrated an excellent detection limit of 0.5 μM, the best among the Hb-based electrochemical biosensors. This is the first electrochemical demonstration of steam-sterilizable, storable, modular bioelectrode that undergoes reversible-thermal denaturation and retains electroactivity for protein based electrochemical applications. MDPI 2015-09-18 /pmc/articles/PMC4610568/ /pubmed/26393601 http://dx.doi.org/10.3390/s150923868 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghimire, Ananta
Zore, Omkar V.
Thilakarathne, Vindya K.
Briand, Victoria A.
Lenehan, Patrick J.
Lei, Yu
Kasi, Rajeswari M.
Kumar, Challa V.
“Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity
title “Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity
title_full “Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity
title_fullStr “Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity
title_full_unstemmed “Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity
title_short “Stable-on-the-Table” Biosensors: Hemoglobin-Poly (Acrylic Acid) Nanogel BioElectrodes with High Thermal Stability and Enhanced Electroactivity
title_sort “stable-on-the-table” biosensors: hemoglobin-poly (acrylic acid) nanogel bioelectrodes with high thermal stability and enhanced electroactivity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610568/
https://www.ncbi.nlm.nih.gov/pubmed/26393601
http://dx.doi.org/10.3390/s150923868
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