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Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia
The objective of this research was to evaluate postmortem changes concerning electric charge of human erythrocytes and thrombocytes in fatal accidental hypothermia. The surface charge density values were determined on the basis of the electrophoretic mobility measurements of the cells conducted at v...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611003/ https://www.ncbi.nlm.nih.gov/pubmed/26364031 http://dx.doi.org/10.1007/s00232-015-9838-1 |
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author | Szeremeta, Michał Petelska, Aneta Dorota Kotyńska, Joanna Pepiński, Witold Naumowicz, Monika Figaszewski, Zbigniew Artur Niemcunowicz-Janica, Anna |
author_facet | Szeremeta, Michał Petelska, Aneta Dorota Kotyńska, Joanna Pepiński, Witold Naumowicz, Monika Figaszewski, Zbigniew Artur Niemcunowicz-Janica, Anna |
author_sort | Szeremeta, Michał |
collection | PubMed |
description | The objective of this research was to evaluate postmortem changes concerning electric charge of human erythrocytes and thrombocytes in fatal accidental hypothermia. The surface charge density values were determined on the basis of the electrophoretic mobility measurements of the cells conducted at various pH values of electrolyte solution. The surface charge of erythrocyte membranes after fatal accidental hypothermia increased compared to the control group within whole range of experimental pH values. Moreover, a slight shift of the isoelectric point of erythrocyte membranes towards high pH values was observed. The surface charge of thrombocyte membranes in fatal accidental hypothermia decreased at low pH compared to the control group. However, at pH range 4–9, the values increased compared to the control group. The isoelectric point of thrombocyte membranes after fatal accidental hypothermia was slightly shifted towards low pH values compared to the control group. The observed changes are probably connected with the partial destruction and functional changes of the blood cell structure. |
format | Online Article Text |
id | pubmed-4611003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-46110032015-10-22 Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia Szeremeta, Michał Petelska, Aneta Dorota Kotyńska, Joanna Pepiński, Witold Naumowicz, Monika Figaszewski, Zbigniew Artur Niemcunowicz-Janica, Anna J Membr Biol Article The objective of this research was to evaluate postmortem changes concerning electric charge of human erythrocytes and thrombocytes in fatal accidental hypothermia. The surface charge density values were determined on the basis of the electrophoretic mobility measurements of the cells conducted at various pH values of electrolyte solution. The surface charge of erythrocyte membranes after fatal accidental hypothermia increased compared to the control group within whole range of experimental pH values. Moreover, a slight shift of the isoelectric point of erythrocyte membranes towards high pH values was observed. The surface charge of thrombocyte membranes in fatal accidental hypothermia decreased at low pH compared to the control group. However, at pH range 4–9, the values increased compared to the control group. The isoelectric point of thrombocyte membranes after fatal accidental hypothermia was slightly shifted towards low pH values compared to the control group. The observed changes are probably connected with the partial destruction and functional changes of the blood cell structure. Springer US 2015-09-12 2015 /pmc/articles/PMC4611003/ /pubmed/26364031 http://dx.doi.org/10.1007/s00232-015-9838-1 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Szeremeta, Michał Petelska, Aneta Dorota Kotyńska, Joanna Pepiński, Witold Naumowicz, Monika Figaszewski, Zbigniew Artur Niemcunowicz-Janica, Anna Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia |
title | Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia |
title_full | Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia |
title_fullStr | Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia |
title_full_unstemmed | Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia |
title_short | Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia |
title_sort | changes in surface charge density of blood cells in fatal accidental hypothermia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611003/ https://www.ncbi.nlm.nih.gov/pubmed/26364031 http://dx.doi.org/10.1007/s00232-015-9838-1 |
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