Cargando…
The oral [(13)C]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios
The oral [(13)C]bicarbonate technique (o(13)CBT) was assessed for the determination of short-term energy expenditure (EE) under field conditions. A total of eight Alaskan huskies were fed two experimental diets in a cross-over experiment including two periods of 3 weeks. Effects of diets on EE, appa...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611086/ https://www.ncbi.nlm.nih.gov/pubmed/26495123 http://dx.doi.org/10.1017/jns.2015.23 |
_version_ | 1782396049053188096 |
---|---|
author | Larsson, Caroline Ahlstrøm, Øystein Junghans, Peter Jensen, Rasmus B. Blache, Dominique Tauson, Anne-Helene |
author_facet | Larsson, Caroline Ahlstrøm, Øystein Junghans, Peter Jensen, Rasmus B. Blache, Dominique Tauson, Anne-Helene |
author_sort | Larsson, Caroline |
collection | PubMed |
description | The oral [(13)C]bicarbonate technique (o(13)CBT) was assessed for the determination of short-term energy expenditure (EE) under field conditions. A total of eight Alaskan huskies were fed two experimental diets in a cross-over experiment including two periods of 3 weeks. Effects of diets on EE, apparent total tract digestibility (ATTD) and on plasma hormones, blood lactate and glucose were furthermore investigated. The percentages of metabolisable energy derived from protein (P), fat (F) and carbohydrates (C) were 26:58:16 in the PFC diet and 24:75:1 in the PF diet. Measurements of EE were performed in the post-absorptive state during rest. Blood samples were collected during rest and exercise and ATTD was determined after days with rest and with exercise. EE was higher (P < 0·01) in period 2 than in period 1 (68 v. 48 kJ/kg body weight(0·75) per h). The ATTD of organic matter, crude protein and crude fat was higher (P < 0·01) in the PF diet compared with the PFC diet, and lower (P < 0·01) for total carbohydrates. Exercise did not affect ATTD. Higher (P < 0·01) insulin-like growth factor 1 and leptin concentrations were measured when fed the PF diet compared with the PFC diet. Concentrations of insulin decreased (P < 0·01), whereas cortisol and ghrelin increased (P < 0·05), after exercise. There was no effect of diet on blood lactate and glucose, but higher (P < 0·001) lactate concentrations were measured in period 1 than in period 2. The results suggest that the o(13)CBT can be used in the field to estimate short-term EE in dogs during resting conditions. Higher ATTD and energy density of the PF diet may be beneficial when energy requirements are high. |
format | Online Article Text |
id | pubmed-4611086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46110862015-10-22 The oral [(13)C]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios Larsson, Caroline Ahlstrøm, Øystein Junghans, Peter Jensen, Rasmus B. Blache, Dominique Tauson, Anne-Helene J Nutr Sci Research Article The oral [(13)C]bicarbonate technique (o(13)CBT) was assessed for the determination of short-term energy expenditure (EE) under field conditions. A total of eight Alaskan huskies were fed two experimental diets in a cross-over experiment including two periods of 3 weeks. Effects of diets on EE, apparent total tract digestibility (ATTD) and on plasma hormones, blood lactate and glucose were furthermore investigated. The percentages of metabolisable energy derived from protein (P), fat (F) and carbohydrates (C) were 26:58:16 in the PFC diet and 24:75:1 in the PF diet. Measurements of EE were performed in the post-absorptive state during rest. Blood samples were collected during rest and exercise and ATTD was determined after days with rest and with exercise. EE was higher (P < 0·01) in period 2 than in period 1 (68 v. 48 kJ/kg body weight(0·75) per h). The ATTD of organic matter, crude protein and crude fat was higher (P < 0·01) in the PF diet compared with the PFC diet, and lower (P < 0·01) for total carbohydrates. Exercise did not affect ATTD. Higher (P < 0·01) insulin-like growth factor 1 and leptin concentrations were measured when fed the PF diet compared with the PFC diet. Concentrations of insulin decreased (P < 0·01), whereas cortisol and ghrelin increased (P < 0·05), after exercise. There was no effect of diet on blood lactate and glucose, but higher (P < 0·001) lactate concentrations were measured in period 1 than in period 2. The results suggest that the o(13)CBT can be used in the field to estimate short-term EE in dogs during resting conditions. Higher ATTD and energy density of the PF diet may be beneficial when energy requirements are high. Cambridge University Press 2015-10-12 /pmc/articles/PMC4611086/ /pubmed/26495123 http://dx.doi.org/10.1017/jns.2015.23 Text en © The Author(s) 2015 http://creativecommons.org/licenses/by/4.0/ This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Larsson, Caroline Ahlstrøm, Øystein Junghans, Peter Jensen, Rasmus B. Blache, Dominique Tauson, Anne-Helene The oral [(13)C]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios |
title | The oral [(13)C]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios |
title_full | The oral [(13)C]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios |
title_fullStr | The oral [(13)C]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios |
title_full_unstemmed | The oral [(13)C]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios |
title_short | The oral [(13)C]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios |
title_sort | oral [(13)c]bicarbonate technique for measurement of short-term energy expenditure of sled dogs and their physiological response to diets with different fat:carbohydrate ratios |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611086/ https://www.ncbi.nlm.nih.gov/pubmed/26495123 http://dx.doi.org/10.1017/jns.2015.23 |
work_keys_str_mv | AT larssoncaroline theoral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT ahlstrømøystein theoral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT junghanspeter theoral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT jensenrasmusb theoral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT blachedominique theoral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT tausonannehelene theoral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT larssoncaroline oral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT ahlstrømøystein oral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT junghanspeter oral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT jensenrasmusb oral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT blachedominique oral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios AT tausonannehelene oral13cbicarbonatetechniqueformeasurementofshorttermenergyexpenditureofsleddogsandtheirphysiologicalresponsetodietswithdifferentfatcarbohydrateratios |