Methylation-Specific PCR Unraveled

Methylation‐specific PCR (MSP) is a simple, quick and cost‐effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whe...

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Detalles Bibliográficos
Autores principales: Derks, Sarah, Lentjes, Marjolein H. F. M., Hellebrekers, Debby M. E. I., de Bruïne, Adriaan P., Herman, James G., van Engeland, Manon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2004
Materias:
Other
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611123/
https://www.ncbi.nlm.nih.gov/pubmed/15623939
http://dx.doi.org/10.1155/2004/370301
Descripción
Sumario:Methylation‐specific PCR (MSP) is a simple, quick and cost‐effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.

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