Methylation-Specific PCR Unraveled

Methylation‐specific PCR (MSP) is a simple, quick and cost‐effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whe...

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Autores principales: Derks, Sarah, Lentjes, Marjolein H. F. M., Hellebrekers, Debby M. E. I., de Bruïne, Adriaan P., Herman, James G., van Engeland, Manon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2004
Materias:
Other
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611123/
https://www.ncbi.nlm.nih.gov/pubmed/15623939
http://dx.doi.org/10.1155/2004/370301
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author Derks, Sarah
Lentjes, Marjolein H. F. M.
Hellebrekers, Debby M. E. I.
de Bruïne, Adriaan P.
Herman, James G.
van Engeland, Manon
author_facet Derks, Sarah
Lentjes, Marjolein H. F. M.
Hellebrekers, Debby M. E. I.
de Bruïne, Adriaan P.
Herman, James G.
van Engeland, Manon
author_sort Derks, Sarah
collection PubMed
description Methylation‐specific PCR (MSP) is a simple, quick and cost‐effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.
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spelling pubmed-46111232016-01-12 Methylation-Specific PCR Unraveled Derks, Sarah Lentjes, Marjolein H. F. M. Hellebrekers, Debby M. E. I. de Bruïne, Adriaan P. Herman, James G. van Engeland, Manon Cell Oncol Other Methylation‐specific PCR (MSP) is a simple, quick and cost‐effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications. IOS Press 2004 2004-12-27 /pmc/articles/PMC4611123/ /pubmed/15623939 http://dx.doi.org/10.1155/2004/370301 Text en Copyright © 2004 Hindawi Publishing Corporation and the authors.
spellingShingle Other
Derks, Sarah
Lentjes, Marjolein H. F. M.
Hellebrekers, Debby M. E. I.
de Bruïne, Adriaan P.
Herman, James G.
van Engeland, Manon
Methylation-Specific PCR Unraveled
title Methylation-Specific PCR Unraveled
title_full Methylation-Specific PCR Unraveled
title_fullStr Methylation-Specific PCR Unraveled
title_full_unstemmed Methylation-Specific PCR Unraveled
title_short Methylation-Specific PCR Unraveled
title_sort methylation-specific pcr unraveled
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611123/
https://www.ncbi.nlm.nih.gov/pubmed/15623939
http://dx.doi.org/10.1155/2004/370301
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AT hellebrekersdebbymei methylationspecificpcrunraveled
AT debruineadriaanp methylationspecificpcrunraveled
AT hermanjamesg methylationspecificpcrunraveled
AT vanengelandmanon methylationspecificpcrunraveled

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