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Cytometric DNA Analysis and Prognostic Biomarkers in Breast Carcinoma. Expression of P53 Product in the Different Ploidy Classes
The aim of the study was to correlate the DNA Index (DI) and S‐phase fraction (SPF) values determined by multiparametric flow cytometry in breast cancer (mainly T1 and T2 stages) with several clinico‐pathologic variables and other biological parameters. For this purpose, a total of 136 breast cancer...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611127/ https://www.ncbi.nlm.nih.gov/pubmed/9373711 http://dx.doi.org/10.1155/1997/345949 |
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author | Leonardi, Elena Cristofori, Antonella Caffo, Orazio Dalla Palma, Paolo |
author_facet | Leonardi, Elena Cristofori, Antonella Caffo, Orazio Dalla Palma, Paolo |
author_sort | Leonardi, Elena |
collection | PubMed |
description | The aim of the study was to correlate the DNA Index (DI) and S‐phase fraction (SPF) values determined by multiparametric flow cytometry in breast cancer (mainly T1 and T2 stages) with several clinico‐pathologic variables and other biological parameters. For this purpose, a total of 136 breast cancers were submitted to flow cytometry and to several types of immunohistochemical analyses. Among clinico‐pathologic data we considered pT, pN and grade and among immunohistochemical markers, hormonal receptors, P53, c‐erbB‐2 and MIB‐1. We found that DNA aneuploidy was strongly correlated with high tumoral grade, absence of hormonal receptors, high proliferation, as shown by high MIB‐1 (≥36%) and SPF values (≥13.3%), and P53 positivity. Grouping the tumours according to their DI values, we observed a relative significantly higher correlation of the near‐triploid range carcinomas (DI 1.40–1.60) with immunohistochemical expression of P53 (p=0.0001). Near‐triploid DI was also associated with a high proliferative activity, expressed both by SPF (p=0.0001) and MIB‐1 reactivity (p=0.0001), high tumoral grade (p=0.0001) and presence of axillary metastases (p=0.03). These data suggest that DNA near‐triploid tumours in breast cancer may have a more aggressive behaviour in comparison with other DI classes. |
format | Online Article Text |
id | pubmed-4611127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46111272016-01-12 Cytometric DNA Analysis and Prognostic Biomarkers in Breast Carcinoma. Expression of P53 Product in the Different Ploidy Classes Leonardi, Elena Cristofori, Antonella Caffo, Orazio Dalla Palma, Paolo Anal Cell Pathol Other The aim of the study was to correlate the DNA Index (DI) and S‐phase fraction (SPF) values determined by multiparametric flow cytometry in breast cancer (mainly T1 and T2 stages) with several clinico‐pathologic variables and other biological parameters. For this purpose, a total of 136 breast cancers were submitted to flow cytometry and to several types of immunohistochemical analyses. Among clinico‐pathologic data we considered pT, pN and grade and among immunohistochemical markers, hormonal receptors, P53, c‐erbB‐2 and MIB‐1. We found that DNA aneuploidy was strongly correlated with high tumoral grade, absence of hormonal receptors, high proliferation, as shown by high MIB‐1 (≥36%) and SPF values (≥13.3%), and P53 positivity. Grouping the tumours according to their DI values, we observed a relative significantly higher correlation of the near‐triploid range carcinomas (DI 1.40–1.60) with immunohistochemical expression of P53 (p=0.0001). Near‐triploid DI was also associated with a high proliferative activity, expressed both by SPF (p=0.0001) and MIB‐1 reactivity (p=0.0001), high tumoral grade (p=0.0001) and presence of axillary metastases (p=0.03). These data suggest that DNA near‐triploid tumours in breast cancer may have a more aggressive behaviour in comparison with other DI classes. IOS Press 1997 1997-01-01 /pmc/articles/PMC4611127/ /pubmed/9373711 http://dx.doi.org/10.1155/1997/345949 Text en Copyright © 1997 Hindawi Publishing Corporation. |
spellingShingle | Other Leonardi, Elena Cristofori, Antonella Caffo, Orazio Dalla Palma, Paolo Cytometric DNA Analysis and Prognostic Biomarkers in Breast Carcinoma. Expression of P53 Product in the Different Ploidy Classes |
title | Cytometric DNA Analysis and Prognostic Biomarkers in Breast Carcinoma. Expression of P53 Product in the Different Ploidy Classes |
title_full | Cytometric DNA Analysis and Prognostic Biomarkers in Breast Carcinoma. Expression of P53 Product in the Different Ploidy Classes |
title_fullStr | Cytometric DNA Analysis and Prognostic Biomarkers in Breast Carcinoma. Expression of P53 Product in the Different Ploidy Classes |
title_full_unstemmed | Cytometric DNA Analysis and Prognostic Biomarkers in Breast Carcinoma. Expression of P53 Product in the Different Ploidy Classes |
title_short | Cytometric DNA Analysis and Prognostic Biomarkers in Breast Carcinoma. Expression of P53 Product in the Different Ploidy Classes |
title_sort | cytometric dna analysis and prognostic biomarkers in breast carcinoma. expression of p53 product in the different ploidy classes |
topic | Other |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611127/ https://www.ncbi.nlm.nih.gov/pubmed/9373711 http://dx.doi.org/10.1155/1997/345949 |
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