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Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/ https://www.ncbi.nlm.nih.gov/pubmed/26539413 http://dx.doi.org/10.3389/fcimb.2015.00074 |
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author | Krüger, Alejandra Lucchesi, Paula M. A. Sanso, A. Mariel Etcheverría, Analía I. Bustamante, Ana V. Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Friedrich, Alexander W. Padola, Nora L. Rossen, John W. A. |
author_facet | Krüger, Alejandra Lucchesi, Paula M. A. Sanso, A. Mariel Etcheverría, Analía I. Bustamante, Ana V. Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Friedrich, Alexander W. Padola, Nora L. Rossen, John W. A. |
author_sort | Krüger, Alejandra |
collection | PubMed |
description | The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx(1a) or stx(2a) subtypes. Interestingly, stx(2a)-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx(2)-positive isolates, whereas katP was only found in stx(1a)-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx(2a). |
format | Online Article Text |
id | pubmed-4612136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46121362015-11-04 Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples Krüger, Alejandra Lucchesi, Paula M. A. Sanso, A. Mariel Etcheverría, Analía I. Bustamante, Ana V. Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Friedrich, Alexander W. Padola, Nora L. Rossen, John W. A. Front Cell Infect Microbiol Microbiology The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx(1a) or stx(2a) subtypes. Interestingly, stx(2a)-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx(2)-positive isolates, whereas katP was only found in stx(1a)-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx(2a). Frontiers Media S.A. 2015-10-20 /pmc/articles/PMC4612136/ /pubmed/26539413 http://dx.doi.org/10.3389/fcimb.2015.00074 Text en Copyright © 2015 Krüger, Lucchesi, Sanso, Etcheverría, Bustamante, Burgán, Fernández, Fernández, Leotta, Friedrich, Padola and Rossen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Krüger, Alejandra Lucchesi, Paula M. A. Sanso, A. Mariel Etcheverría, Analía I. Bustamante, Ana V. Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Friedrich, Alexander W. Padola, Nora L. Rossen, John W. A. Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title | Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_full | Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_fullStr | Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_full_unstemmed | Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_short | Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples |
title_sort | genetic characterization of shiga toxin-producing escherichia coli o26:h11 strains isolated from animal, food, and clinical samples |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/ https://www.ncbi.nlm.nih.gov/pubmed/26539413 http://dx.doi.org/10.3389/fcimb.2015.00074 |
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