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Influence of P53 on the radiotherapy response of hepatocellular carcinoma

BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and it has a poor prognosis and few therapeutic options. Radiotherapy is one of the most effective forms of cancer treatment, and P53 protein is one of the key molecules determining how a cell responds to ra...

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Autores principales: Gomes, Ana R., Abrantes, Ana M., Brito, Ana F., Laranjo, Mafalda, Casalta-Lopes, João E., Gonçalves, Ana C., Sarmento-Ribeiro, Ana B., Botelho, Maria F., Tralhão, José G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Association for the Study of the Liver 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612287/
https://www.ncbi.nlm.nih.gov/pubmed/26527121
http://dx.doi.org/10.3350/cmh.2015.21.3.257
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author Gomes, Ana R.
Abrantes, Ana M.
Brito, Ana F.
Laranjo, Mafalda
Casalta-Lopes, João E.
Gonçalves, Ana C.
Sarmento-Ribeiro, Ana B.
Botelho, Maria F.
Tralhão, José G.
author_facet Gomes, Ana R.
Abrantes, Ana M.
Brito, Ana F.
Laranjo, Mafalda
Casalta-Lopes, João E.
Gonçalves, Ana C.
Sarmento-Ribeiro, Ana B.
Botelho, Maria F.
Tralhão, José G.
author_sort Gomes, Ana R.
collection PubMed
description BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and it has a poor prognosis and few therapeutic options. Radiotherapy is one of the most effective forms of cancer treatment, and P53 protein is one of the key molecules determining how a cell responds to radiotherapy. The aim of this study was to determine the therapeutic efficacy of iodine-131 in three human HCC cell lines. METHODS: Western blotting was used to measure P53 expression. The effects of radiotherapy with iodine-131 were assessed by using the clonogenic assay to evaluate cell survival. Flow cytometry was carried out to examine the effects of iodine-131 on cell death, oxidative stress, reduced intracellular glutathione expression, the mitochondrial membrane potential, and the cell cycle. RESULTS: The P53 protein was not expressed in Hep3B2.1-7 cells, was expressed at normal levels in HepG2 cells, and was overexpressed in HuH7 cells. P53 expression in the HuH7 and HepG2 cell lines increased after internal and external irradiation with iodine-131. Irradiation induced a decrease in cell survival and led to a decrease in cell viability in all of the cell lines studied, accompanied by cell death via late apoptosis/necrosis and necrosis. Irradiation with 131-iodine induced mostly cell-cycle arrest in the G0/G1 phase. CONCLUSIONS: These results suggest that P53 plays a key role in the radiotherapy response of HCC.
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spelling pubmed-46122872015-10-30 Influence of P53 on the radiotherapy response of hepatocellular carcinoma Gomes, Ana R. Abrantes, Ana M. Brito, Ana F. Laranjo, Mafalda Casalta-Lopes, João E. Gonçalves, Ana C. Sarmento-Ribeiro, Ana B. Botelho, Maria F. Tralhão, José G. Clin Mol Hepatol Original Article BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and it has a poor prognosis and few therapeutic options. Radiotherapy is one of the most effective forms of cancer treatment, and P53 protein is one of the key molecules determining how a cell responds to radiotherapy. The aim of this study was to determine the therapeutic efficacy of iodine-131 in three human HCC cell lines. METHODS: Western blotting was used to measure P53 expression. The effects of radiotherapy with iodine-131 were assessed by using the clonogenic assay to evaluate cell survival. Flow cytometry was carried out to examine the effects of iodine-131 on cell death, oxidative stress, reduced intracellular glutathione expression, the mitochondrial membrane potential, and the cell cycle. RESULTS: The P53 protein was not expressed in Hep3B2.1-7 cells, was expressed at normal levels in HepG2 cells, and was overexpressed in HuH7 cells. P53 expression in the HuH7 and HepG2 cell lines increased after internal and external irradiation with iodine-131. Irradiation induced a decrease in cell survival and led to a decrease in cell viability in all of the cell lines studied, accompanied by cell death via late apoptosis/necrosis and necrosis. Irradiation with 131-iodine induced mostly cell-cycle arrest in the G0/G1 phase. CONCLUSIONS: These results suggest that P53 plays a key role in the radiotherapy response of HCC. The Korean Association for the Study of the Liver 2015-09 2015-09-30 /pmc/articles/PMC4612287/ /pubmed/26527121 http://dx.doi.org/10.3350/cmh.2015.21.3.257 Text en Copyright © 2015 by The Korean Association for the Study of the Liver http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Gomes, Ana R.
Abrantes, Ana M.
Brito, Ana F.
Laranjo, Mafalda
Casalta-Lopes, João E.
Gonçalves, Ana C.
Sarmento-Ribeiro, Ana B.
Botelho, Maria F.
Tralhão, José G.
Influence of P53 on the radiotherapy response of hepatocellular carcinoma
title Influence of P53 on the radiotherapy response of hepatocellular carcinoma
title_full Influence of P53 on the radiotherapy response of hepatocellular carcinoma
title_fullStr Influence of P53 on the radiotherapy response of hepatocellular carcinoma
title_full_unstemmed Influence of P53 on the radiotherapy response of hepatocellular carcinoma
title_short Influence of P53 on the radiotherapy response of hepatocellular carcinoma
title_sort influence of p53 on the radiotherapy response of hepatocellular carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612287/
https://www.ncbi.nlm.nih.gov/pubmed/26527121
http://dx.doi.org/10.3350/cmh.2015.21.3.257
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