Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae

Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of...

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Autores principales: Qi, Xin, Zha, Jian, Liu, Gao-Gang, Zhang, Weiwen, Li, Bing-Zhi, Yuan, Ying-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612707/
https://www.ncbi.nlm.nih.gov/pubmed/26539187
http://dx.doi.org/10.3389/fmicb.2015.01165
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author Qi, Xin
Zha, Jian
Liu, Gao-Gang
Zhang, Weiwen
Li, Bing-Zhi
Yuan, Ying-Jin
author_facet Qi, Xin
Zha, Jian
Liu, Gao-Gang
Zhang, Weiwen
Li, Bing-Zhi
Yuan, Ying-Jin
author_sort Qi, Xin
collection PubMed
description Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of XylA and endogenous XKS1. Additional overexpression of genes RKI1, RPE1, TKL1, and TAL1 in the non-oxidative pentose phosphate pathway (PPP) in SyBE001 increased the xylose consumption rate by 1.19-fold. By repetitive adaptation, the xylose utilization rate was further increased by ∼10-fold in the resultant strain SyBE003. Gene expression analysis identified a variety of genes with significantly changed expression in the PPP, glycolysis and the tricarboxylic acid cycle in SyBE003.
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spelling pubmed-46127072015-11-04 Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae Qi, Xin Zha, Jian Liu, Gao-Gang Zhang, Weiwen Li, Bing-Zhi Yuan, Ying-Jin Front Microbiol Microbiology Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of XylA and endogenous XKS1. Additional overexpression of genes RKI1, RPE1, TKL1, and TAL1 in the non-oxidative pentose phosphate pathway (PPP) in SyBE001 increased the xylose consumption rate by 1.19-fold. By repetitive adaptation, the xylose utilization rate was further increased by ∼10-fold in the resultant strain SyBE003. Gene expression analysis identified a variety of genes with significantly changed expression in the PPP, glycolysis and the tricarboxylic acid cycle in SyBE003. Frontiers Media S.A. 2015-10-21 /pmc/articles/PMC4612707/ /pubmed/26539187 http://dx.doi.org/10.3389/fmicb.2015.01165 Text en Copyright © 2015 Qi, Zha, Liu, Zhang, Li and Yuan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Qi, Xin
Zha, Jian
Liu, Gao-Gang
Zhang, Weiwen
Li, Bing-Zhi
Yuan, Ying-Jin
Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
title Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
title_full Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
title_fullStr Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
title_full_unstemmed Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
title_short Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
title_sort heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in saccharomyces cerevisiae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612707/
https://www.ncbi.nlm.nih.gov/pubmed/26539187
http://dx.doi.org/10.3389/fmicb.2015.01165
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