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CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of “Wuzishatangju” (Citrus reticulata Blanco)

Plant S-phase kinase-associated protein 1 (SKP1) genes play crucial roles in plant development and differentiation. However, the role of SKP1 in citrus is unclear. Herein, we described a novel SKP1-like gene, designated as CrWSKP1, from “Wuzishatangju” (Citrus reticulata Blanco). The cDNA sequence o...

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Detalles Bibliográficos
Autores principales: Li, Peng, Miao, Hongxia, Ma, Yuewen, Wang, Lu, Hu, Guibing, Ye, Zixing, Zhao, Jietang, Qin, Yonghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613275/
https://www.ncbi.nlm.nih.gov/pubmed/26370985
http://dx.doi.org/10.3390/ijms160921695
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author Li, Peng
Miao, Hongxia
Ma, Yuewen
Wang, Lu
Hu, Guibing
Ye, Zixing
Zhao, Jietang
Qin, Yonghua
author_facet Li, Peng
Miao, Hongxia
Ma, Yuewen
Wang, Lu
Hu, Guibing
Ye, Zixing
Zhao, Jietang
Qin, Yonghua
author_sort Li, Peng
collection PubMed
description Plant S-phase kinase-associated protein 1 (SKP1) genes play crucial roles in plant development and differentiation. However, the role of SKP1 in citrus is unclear. Herein, we described a novel SKP1-like gene, designated as CrWSKP1, from “Wuzishatangju” (Citrus reticulata Blanco). The cDNA sequence of CrWSKP1 is 779 base pairs (bp) and contains an open reading frame (ORF) of 477 bp. The genomic sequence of the CrWSKP1 gene is 1296 bp with two exons and one intron. CrWSKP1 has high identity with SKP1-like genes from other plant species within two conserved regions. Approximately 85% of pollen tubes of self-pollinated CrWSKP1 transgenic tobaccos became twisted at four days after self-pollination. Pollen tube numbers of self-pollinated CrWSKP1 transformants entering into ovules were significantly fewer than that of the control. Seed number of self-pollinated CrWSKP1 transformants was significantly reduced. These results suggested that the CrWSKP1 is involved in the self-incompatibility (SI) reaction of “Wuzishatangju”.
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spelling pubmed-46132752015-10-26 CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of “Wuzishatangju” (Citrus reticulata Blanco) Li, Peng Miao, Hongxia Ma, Yuewen Wang, Lu Hu, Guibing Ye, Zixing Zhao, Jietang Qin, Yonghua Int J Mol Sci Article Plant S-phase kinase-associated protein 1 (SKP1) genes play crucial roles in plant development and differentiation. However, the role of SKP1 in citrus is unclear. Herein, we described a novel SKP1-like gene, designated as CrWSKP1, from “Wuzishatangju” (Citrus reticulata Blanco). The cDNA sequence of CrWSKP1 is 779 base pairs (bp) and contains an open reading frame (ORF) of 477 bp. The genomic sequence of the CrWSKP1 gene is 1296 bp with two exons and one intron. CrWSKP1 has high identity with SKP1-like genes from other plant species within two conserved regions. Approximately 85% of pollen tubes of self-pollinated CrWSKP1 transgenic tobaccos became twisted at four days after self-pollination. Pollen tube numbers of self-pollinated CrWSKP1 transformants entering into ovules were significantly fewer than that of the control. Seed number of self-pollinated CrWSKP1 transformants was significantly reduced. These results suggested that the CrWSKP1 is involved in the self-incompatibility (SI) reaction of “Wuzishatangju”. MDPI 2015-09-09 /pmc/articles/PMC4613275/ /pubmed/26370985 http://dx.doi.org/10.3390/ijms160921695 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Peng
Miao, Hongxia
Ma, Yuewen
Wang, Lu
Hu, Guibing
Ye, Zixing
Zhao, Jietang
Qin, Yonghua
CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of “Wuzishatangju” (Citrus reticulata Blanco)
title CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of “Wuzishatangju” (Citrus reticulata Blanco)
title_full CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of “Wuzishatangju” (Citrus reticulata Blanco)
title_fullStr CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of “Wuzishatangju” (Citrus reticulata Blanco)
title_full_unstemmed CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of “Wuzishatangju” (Citrus reticulata Blanco)
title_short CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of “Wuzishatangju” (Citrus reticulata Blanco)
title_sort crwskp1, an skp1-like gene, is involved in the self-incompatibility reaction of “wuzishatangju” (citrus reticulata blanco)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613275/
https://www.ncbi.nlm.nih.gov/pubmed/26370985
http://dx.doi.org/10.3390/ijms160921695
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